| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Genome editing techniques such as CRISPR/Cas9 system enable us to analyze the gene function. However, production of F1 (sometimes F2) generation is necessary for confirming the gene function by repetitive analyses because the state of mutations is different among the genome-edited individuals even if they are produced by the same procedure. In animals that need long periods for becoming sexually mature, we have to take long time to get population having the same mutation in the target gene. To overcome this disadvantage, I produced clonal population of genome-edited Xenopus laevis by transplantation of nucleus from embryo genome-edited by CRISPR/Cas9 system into enucleated egg. In addition, I succeeded in transplantation of nucleus from embryo which had been cryopreserved by vitrification. This technique allows us not only to ensure a sufficient time for selection of embryo having the desired mutation in the target gene but also to keep the useful animal in the narrow space.
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