Light-induced regulation of flagellar and ciliary motility by a novel photoreceptor protein

• Project/Area Number: 15K14566
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Animal physiology/Animal behavior
• Research Institution: University of Tsukuba
• Principal Investigator: INABA Kazuo 筑波大学, 生命環境系, 教授 (80221779)
• Co-Investigator(Renkei-kenkyūsha): SHIBA Kogiku 筑波大学, 生命環境系, 助教 (70533561) ; HIROSE Keiko 国立研究開発法人産業総合研究所, バイオメディカル研究部門, 主任研究員 (90357872)
• Research Collaborator: KUTOMI Osamu ; JOKURA Kei
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: DYBLUP / ダイニン / BLUP / 光応答 / 鞭毛 / 繊毛 / 精子 / クラミドモナス / 青色光 / 光受容 / 繊毛運動 / ホヤ / 微小管

Outline of Final Research Achievements

This research project was carried out to clarify the molecular nature and mechanism of a novel dynein photoreceptor DYBLUP. The 2 years project showed (1) the molecule of f/I1 dynein form ascidian sperm is similar to that from Chlamydomonas flagella, (2) ascidian DYBLUP is bound to the motor region of f/I1, (3) the protein product of a gene (MOT7) orthologous to DYBLUP is also present in Chlamydomonas flagella, (5) a Chlamydomonas mutant lacking DYBLUP/MOT7 shows suppressed positive phototaxis and the turn movement in photoshock reaction.

Research Products

• [Journal Article] Cooperative Wnt-Nodal signals regulate the patterning of anterior neuroectoderm. (2016)
  • Author(s): Yaguchi J, Takeda N, Inaba K, Yaguchi S
  • Journal Title: PLoS Genetics Volume: 12(4) Issue: 4 Pages: 1-27
  • DOI: 10.1371/journal.pgen.1006001
  • NAID: 120007135521
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Calcium sensors of ciliary outer arm dynein: functions and phylogenetic consideration for eukaryotic evolution (2015)
  • Author(s): Inaba K.
  • Journal Title: Cilia Volume: in press Issue: 1 Pages: 6-6
  • DOI: 10.1186/s13630-015-0015-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Branchial cilia and sperm flagella recruit distinct axonemal components (2015)
  • Author(s): Konno A, Shiba K, Cai C, Inaba K
  • Journal Title: PLoS One Volume: 10 Issue: 5 Pages: 10-10
  • DOI: 10.1371/journal.pone.0126005
  • Peer Reviewed / Open Access
• [Presentation] A novel subunit of axonemal dynein contains a photoreceptor protein domain (2016)
  • Author(s): Osamu Kutomi, Keiko Hirose, Katsutoshi Mizuno, Kogiku Shiba, Lixy Yamada, Hitoshi Sawada, Daisuke Shibata, Ryosuke Yamamoto, Kazuo Inaba
  • Organizer: 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research / Invited
• [Presentation] Isolation of a non-phototactic Chlamydomonas mutant that shows alternate flagellar beatings (2016)
  • Author(s): Akinori Koitabashi, Masako Nakajima, Noriko Ueki, Kogiku Shiba, Takashi Yamano, Hiro Iguchi, Haruka Arakawa, Yuri Fukuda, Hideya Fukuzawa, Kazuo Inaba, Toru Hisabori, Ken-ichi Wakabayashi
  • Organizer: 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] カタユウレイボヤ精子鞭毛における内腕ダイニンのキャラクタリゼーション (2015)
  • Author(s): Osamu Kutomi, Katsutoshi Mizuno, Keiko Hirose, Lixy Yamada, Hitoshi Sawada, Kogiku Shiba, Kazuo Inaba
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学（石川県・金沢市）
  • Year and Date: 2015-09-13
• [Presentation] Calcium sensors of ciliary outer arm dynein and eukaryotic evolution (2015)
  • Author(s): Kazuo Inaba
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟コンベンションセンター（新潟県・新潟市）
  • Year and Date: 2015-09-07
  • Invited
• [Presentation] 海の生き物のミクロな動き (2015)
  • Author(s): 稲葉 一男
  • Organizer: 第47回知の拠点セミナー
  • Place of Presentation: 京都大学東京オフィス（東京都・港区）
  • Year and Date: 2015-08-21
  • Invited
• [Presentation] Two novel subunits of axonemal dyneins from Ciona intestinalis, as potentially key regulators for ciliary motility (2015)
  • Author(s): Kazuo Inaba, Osamu Kutomi, Katsutoshi Mizuno, Kogiku Shiba, Keiko Hirose
  • Organizer: The 8th International Tunicate Meeting
  • Place of Presentation: Aomori Civic Cultural Hall, Aomori, Japan
  • Year and Date: 2015-07-13
  • Int'l Joint Research
• [Book] Flagellar Mechanics and Sperm Guidance (2015)
  • Author(s): Inaba K, Kutomi O, Shiba K, Cosson J.
  • Total Pages: 50
  • Publisher: Bentham Science Publishers
• [Remarks] 筑波大学下田臨海実験センター稲葉研究室研究紹介
  • URL: http://www.shimoda.tsukuba.ac.jp/~inaba/publish.html
• [Remarks] 筑波大学下田臨海実験センター稲葉研究室
  • URL: http://www.shimoda.tsukuba.ac.jp/~inaba/