Neural mechanism for integration of vector information encoded in honeybee waggle dance

• Project/Area Number: 15K14569
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Animal physiology/Animal behavior
• Research Institution: Fukuoka University
• Principal Investigator: Ai Hiroyuki 福岡大学, 理学部, 助教 (20330897)
• Co-Investigator(Kenkyū-buntansha): 池野 英利 兵庫県立大学, 環境人間学部, 教授 (80176114)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 脳・神経 / 行動学 / 神経科学 / 昆虫 / 生理学

Outline of Final Research Achievements

The follower of honeybee waggle dance decipher the distance information to the profitable flower from the duration of waggle phase. On this study the morphological and electrophysiological properties of vibration sensitive interneuron in the primary auditory center in the brain have been analyzed in responding to the waggle dance sound mimicked vibration pulse stimuli to the antenna and its possible neural circuit was suggested by experimental and theoretical evidences. DL-Int-1 has spontaneous activities and shows a tonic inhibition during waggle dance sound mimicked vibration pulse stimuli. Anti-GABA immunohistochemical experiment suggested that D-Int-1 is a GABAergic inhibitory neuron. The putative postsynaptic neuron DL-Int-2 shows a tonic excitation during the waggle dance sound mimicked vibration pulse stimuli. These results suggest that disinhibition from the sensory neuron to the DL-Int-2 through DL-Int-1 might be a key neural network to encode the distance information.

Research Products

• [Int'l Joint Research] Ludwig-Maximilians- Universitat Munchen(Germany)
• [Int'l Joint Research] Ludwig Maximillian Univ. Munich(ドイツ)
• [Int'l Joint Research] Ludwig Maximillian Univ. Munich(ドイツ)
• [Journal Article] Spatial registration of neuron morphologies based on maximization of volume overlap (2018)
  • Author(s): Kumaraswamy Ajayrama、Kai Kazuki、Ai Hiroyuki、Ikeno Hidetoshi、Wachtler Thomas
  • Journal Title: BMC Bioinformatics Volume: 19 Issue: 1 Pages: 1-16
  • DOI: 10.1186/s12859-018-2136-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ミツバチ脳のリバースエンジニアリング －ダンスコミュニケーションに関わる神経機構－ (2018)
  • Author(s): 池野 英利, 藍 浩之
  • Journal Title: 昆虫と自然 Volume: 53 (6) Pages: 35-40
  • Peer Reviewed
• [Journal Article] Development of Behavior Monitoring System for Honeybees in Hive (2017)
  • Author(s): 高橋伸弥・橋本浩二・前田佐嘉志・鶴田直之・藍浩之
  • Journal Title: Transactions of the Japanese Society for Artificial Intelligence Volume: 32 Issue: 4 Pages: B-GC2_1-11
  • DOI: 10.1527/tjsai.B-GC2
  • NAID: 130006887097
  • ISSN: 1346-0714, 1346-8030
  • Year and Date: 2017-07-01
  • Peer Reviewed / Open Access
• [Journal Article] Interneurons in the honeybee primary auditory center responding to waggle dance-like vibration pulses (2017)
  • Author(s): Hiroyuki Ai, Kazuki Kai, Ajayrama Kumaraswamy, Hidetoshi Ikeno, Thomas Wachtler
  • Journal Title: Journal of Neuroscience Volume: 37(44) Issue: 44 Pages: 10624-10635
  • DOI: 10.1523/jneurosci.0044-17.2017
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Phototactic behavior of the ambrosia beetle Platypus quercivorus (Murayama) (Coleoptera: Platypodidae) before and after flight (2017)
  • Author(s): Long Pham Duy, Yasuto Ito, Ryuichi Okada, Hidetoshi Ikeno, Yuji Isagi, Michimasa Yamasaki
  • Journal Title: Journal of Insect Behavior Volume: 30 Issue: 3 Pages: 318-330
  • DOI: 10.1007/s10905-017-9615-3
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] ミツバチコロニーの巣内行動観察システムの開発―RFIDセンサと画像処理を併用したコミュニケ―ション行動の自動検出― (2017)
  • Author(s): 高橋伸弥、橋本浩二、前田佐嘉志、鶴田直之、藍浩之
  • Journal Title: 人工知能学会論文誌 Volume: 印刷中
  • NAID: 130006887097
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Walking patterns induced by learned odors in the honeybee, Apis mellifera L. (2016)
  • Author(s): Yamashita, T., Haupt, S. S., Ikeno, H., Ai, H.
  • Journal Title: Journal of Experimental Biology Volume: 219 Pages: 12-16
  • DOI: 10.1242/jeb.123356
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ミツバチのダンス言語の発達の過程 ―RFIDを用いた羽化後20日間の行動観察― (2018)
  • Author(s): 藍浩之
  • Organizer: 第40回ミツバチ科学研究会
  • Invited
• [Presentation] Interneurons in the primary auditory center of the honeybee brain responsive to air vibration pulses as elicited during waggle dance communication (2017)
  • Author(s): Wachtler, T, Kumaraswamy, A, Kai, K., Ikeno, H., Ai, H.
  • Organizer: Society for Neuroscience 2017
  • Int'l Joint Research
• [Presentation] ミツバチの尻振りダンスコミュニケーションにおける蜜源への距離情報の符号化回路 (2017)
  • Author(s): 藍浩之
  • Organizer: 日本動物学会第88回富山大会
  • Invited
• [Presentation] Development of honeybee wagle dance and its differences between recruits and scouts (2017)
  • Author(s): Hiroyuki Ai, Yuuki Kobayashi, Toshiyuki Matake, Shinya Takahashi, Koji Hashimoto, Sakashi Maeda, Naoyuki Tsuruta.
  • Organizer: JSCPB 2017 Fukuoka
• [Presentation] 二ホンミツバチにおける女王パイピング（トゥーティング、クワッキング）の特徴および分蜂との関係 (2017)
  • Author(s): 山本哲、菅原道夫、池野英利、岡田龍一
  • Organizer: 日本動物学会第88回富山大会
• [Presentation] ダンス追従バチは複数のダンス蜂を追従する (2017)
  • Author(s): 岡田龍一、池野英利、青沼仁志
  • Organizer: 日本動物学会第88回富山大会
• [Presentation] 自律分散システムとしてのミツバチ：集団を支える自律分散機構 (2017)
  • Author(s): 池野英利
  • Organizer: 計測自動制御学会第60回自律分散システム部会研究会
  • Invited
• [Presentation] ミツバチの個性と役割分化：コミュニケーションの視点から－ (2017)
  • Author(s): 藍浩之
  • Organizer: 計測自動制御学会第60回自律分散システム部会研究会
  • Invited
• [Presentation] Automatic tracking method for multiple honeybees using backward-play movies (2017)
  • Author(s): T. Kimura, M. Ohashi, R. Okada, K. Crailsheim, T. Schmickl, G. Radspieler, T. Isokawa, H. Ikeno
  • Organizer: ICIEV-ISCMHT
  • Int'l Joint Research
• [Presentation] Virtual computing server for trial use of mathematical model and data analysis software：Sim-P (2017)
  • Author(s): Hidetoshi Ikeno
  • Organizer: NIX-odML Global Workshop and Hackathon 2017 in Japan
  • Int'l Joint Research / Invited
• [Presentation] Development of a hypertext tutorial for construction of a standard brain (2017)
  • Author(s): Hidetoshi Ikeno
  • Organizer: Advances in Neuroinformatics V
  • Int'l Joint Research / Invited
• [Presentation] RFIDと高解像度ビデオ記録を用いたミツバチ行動自動追跡システムの開発 (2017)
  • Author(s): 藍浩之、高橋伸弥、橋本浩二、前田佐嘉志、鶴田直之
  • Organizer: 第6回ミツバチシンポジウム
  • Place of Presentation: 福岡
• [Presentation] 尻振りダンス発現までのダンスコミュニケーションの解析 (2017)
  • Author(s): 小林裕貴、田中聡至、牛島実佑、楠目晃大、目崇志、藍浩之
  • Organizer: 第6回ミツバチシンポジウム
  • Place of Presentation: 福岡
• [Presentation] セイヨウミツバチの自動計数システム (2017)
  • Author(s): 野口 凌, 礒川 悌次郎, 池野 英利, 峯本 俊文, 松井 伸之
  • Organizer: 第6回ミツバチシンポジウム
  • Place of Presentation: 福岡
• [Presentation] ミツバチコロニーのエネルギー収支の季節変化-成長期と退行期の比較 (2017)
  • Author(s): 吉田澪、大橋瑞江、木村敏文、岡田龍一、池野英利
  • Organizer: 第6回ミツバチシンポジウム
  • Place of Presentation: 福岡
• [Presentation] ミツバチコロニーの成長と退行特性に関する数理モデル解析 (2017)
  • Author(s): 甘中健一、池野英利、岡田龍一、木村敏文、大橋瑞江
  • Organizer: 第6回ミツバチシンポジウム
  • Place of Presentation: 福岡
• [Presentation] Putative neural circuits of the primary auditory center processing in the pulsed vibration produced by waggle dance of honeybee (2016)
  • Author(s): Ai, H., Kai, K., Kumaraswamy, A, Rautenberg, P. Wachtler, T, Ikeno, H. Ai.
  • Organizer: The 22nd International Congress of Zoology
  • Place of Presentation: Okinawa
  • Year and Date: 2016-11-14
  • Int'l Joint Research / Invited
• [Presentation] Lifetime observation of the honeybee behaviors related with waggle dance by using RFIDs and high resolution camera modules. (2016)
  • Author(s): Ai, H., Matake, T., Hagio, H., Takahashi, S., Hashimoto, K., Maeda, S., Trusuta, N.
  • Organizer: The 22nd International Congress of Zoology
  • Place of Presentation: Okinawa
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Development of automatic monitoring system of honeybee behaviors using RFID reader and imaging processing. (2016)
  • Author(s): Takahashi, S., Hashimoto, K., Maeda, S., Trusuta, N., Ai, H.
  • Organizer: The 22nd International Congress of Zoology
  • Place of Presentation: Okinawa
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Morphological comparisons in a vibration sensitive interneuron in the dorsal lobe of the brain among honeybees of different ages (2016)
  • Author(s): Hidetoshi Ikeno, Ajayrama Kumaraswamy, Kazuki Kai, Hiroyuki Ai, Philipp Rautenberg, Thomas Wachtler
  • Organizer: International Conference on Brain Informatics & Health
  • Place of Presentation: Omaha, Nebraska, USA
  • Year and Date: 2016-10-13
  • Int'l Joint Research
• [Presentation] ARタグを用いたミツバチ歩行軌跡同時追跡アルゴリズムの検討 (2016)
  • Author(s): 高橋伸弥、橋本浩二、前田佐嘉志、鶴田直之、藍浩之
  • Organizer: FIT2016 (第15回情報科学技術フォーラム)
  • Place of Presentation: 富山
  • Year and Date: 2016-09-07
• [Presentation] A method for evaluation of neural structure based on reconstruction and application to an interneuron in the honey bee brain (2016)
  • Author(s): Hidetoshi Ikeno, Ajayrama Kumaraswamy, Kazuki Kai, Hiroyuki Ai, Philipp Rautenberg, Thomas Wachtler
  • Organizer: INCF Congress 2016
  • Place of Presentation: Reading, UK
  • Year and Date: 2016-09-03
  • Int'l Joint Research
• [Presentation] Interneurons of the primary auditory center of the honeybee brain responsive to pulsed vibration produced by the waggle dance. (2016)
  • Author(s): Ai, H., Kai, K., Kumaraswamy, A, Rautenberg, P. Wachtler, T, Ikeno, H. Ai, H.
  • Organizer: JSCPB 2016 (The 38th Annual meeting of the Japanese Society for Comparative Physiology and Biochemistry)
  • Place of Presentation: Tokyo
  • Year and Date: 2016-09-02
• [Presentation] Honeybee neurons responsive to the pulsed vibration produced by waggle dance (2016)
  • Author(s): Ai, H., Kai, K., Watanabe, H., Itoh, T., Kumaraswamy, A, Rautenberg, P. Wachtler, T, Ikeno, H.
  • Organizer: 4th INCF Japan Node International Workshop Advances in Neuroinformatics 2016 and 14th INCF Nodes Workshop
  • Place of Presentation: Saitama
  • Year and Date: 2016-05-28
  • Int'l Joint Research
• [Presentation] Neuron segmentation plugin for Vaa3D and its application to morphometric analysis of an interneuron in the honeybee brain (2016)
  • Author(s): Hidetoshi Ikeno
  • Organizer: BigNeuron and Big Neuroscience Workshop
  • Place of Presentation: Nanjing, China
  • Year and Date: 2016-05-26
  • Int'l Joint Research
• [Presentation] Honeybee interneurons responsive to the pulsed vibration produced by waggle dance (2016)
  • Author(s): Ai, H., Kai, K., Watanabe, H., Itoh, T., Kumaraswamy, A, Rautenberg, P. Wachtler, T, Ikeno, H.
  • Organizer: International congress of neuroethology 2016
  • Place of Presentation: Montevideo, Uruguay
  • Year and Date: 2016-03-30
  • Int'l Joint Research / Invited
• [Presentation] ミツバチの採餌行動におけるエネルギー収支 (2016)
  • Author(s): 澤井穂高，岡田龍一,吉田澪,大橋瑞江，木村敏文，池野英利
  • Organizer: 日本生態学会第63回全国大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-03-22
• [Presentation] Seasonal changes of energy gain and consumption in a honeybee colony (2016)
  • Author(s): YOSHIDA,M.,OHASHI,M.,KIMURA,T.,OKADA,R.,IKENO,H.
  • Organizer: 日本生態学会第63回全国大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-03-22
• [Presentation] RFIDと小型HDカメラモジュールを用いたミツバチの尻振りダンス関連行動の解析 (2016)
  • Author(s): 藍 浩之, 高橋伸弥、橋本浩二、前田佐嘉志、鶴田直之
  • Organizer: 第5回ミツバチシンポジウム
  • Place of Presentation: 姫路
  • Year and Date: 2016-02-19
  • Invited
• [Presentation] Comprehensive analyses of interneurons arborizing in the primary vibration center in honeybee brain. (2015)
  • Author(s): Ai, H., Kai, K., Watanabe, H., Kumaraswamy, A, Rautenberg, P. Wachtler, T, Ikeno, H. Ai, H.
  • Organizer: CompBiol 2015
  • Place of Presentation: 広島
  • Year and Date: 2015-12-11
• [Presentation] A method for construction and application of standard brain model based on the confocal images (2015)
  • Author(s): H. Ikeno, D. Miyamoto, T. Kazawa, R. Kanzaki,
  • Organizer: Advances in Neuroinformatics2015 tutorial session
  • Place of Presentation: 東京
  • Year and Date: 2015-11-27
  • Invited
• [Presentation] Development of protocol and software for registration in situ hybridization image into the standard brain (2015)
  • Author(s): H. Ikeno, D. Miyamoto, Y. Okamura-Oho, H. Nishibe, A. Sato, T. Furuichi, R. Kanzaki
  • Organizer: Advances in Neuroinformatics 2015
  • Place of Presentation: 東京
  • Year and Date: 2015-11-27
  • Invited
• [Presentation] 学習した匂いと尻振りダンス振動によって誘発されるミツバチの歩行の解析 (2015)
  • Author(s): 山下俊也、池野英利、長谷川雄二、伊東綱男、藍 浩之
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-17
• [Presentation] ツバチの出巣・帰巣モニタリングのためのアレイ型赤外線素子を用いた熱画像撮影装置の開発 (2015)
  • Author(s): 橋本浩二、前田佐嘉志、藍 浩之
  • Organizer: バイオイメージ・インフォマティクスワークショップ2015
  • Place of Presentation: 福岡
  • Year and Date: 2015-06-18
• [Presentation] ミツバチ歩行軌跡の複数個体同時追跡アルゴリズムを用いた尻振りダンス軌跡の抽出 (2015)
  • Author(s): 高橋伸弥、井出翔太、鶴田直之、藍 浩之
  • Organizer: 人工知能学会全国大会2015
  • Place of Presentation: 函館
  • Year and Date: 2015-05-30
• [Presentation] ミツバチにおける学習した匂いによって誘発される歩行の経時変化と音刺激による影響 (2015)
  • Author(s): 山下俊也、Haupt, S.S.、池野英利、伊東綱男、藍 浩之
  • Organizer: 日本動物学会九州支部第68回大会（三学会合同福岡大会）
  • Place of Presentation: 福岡
  • Year and Date: 2015-05-23
• [Book] 脳地図作成の概要とソフトウェア（昆虫の脳をつくる、第8章） (2018)
  • Author(s): 池野英利、岩月知香、神崎亮平
  • Total Pages: 11
  • Publisher: 朝倉書店
• [Book] 標準脳作成の実際（昆虫の脳を作る、第9章） (2018)
  • Author(s): 池野英利、岩月知香、後藤昴彦、宮本大輔、神崎亮平
  • Total Pages: 39
  • Publisher: 朝倉書店
• [Book] 虫の鼻はどこ？電気で測る触角の働き―カイコガのフェロモン腺の観察と触角電図による匂い応答解析―、研究者が教える動物実験 第1巻 (2015)
  • Author(s): 藍 浩之
  • Total Pages: 4
  • Publisher: 共立出版
• [Book] パブロフのミツバチ―餌の匂いはどれ？―、研究者が教える動物実験 第3巻 (2015)
  • Author(s): 佐倉 緑、岡田龍一、藍 浩之
  • Total Pages: 4
  • Publisher: 共立出版
• [Book] ダンゴムシのジグザグ歩行―迷路を用いた交替性転向反応の観察とその仕組みの検討―、研究者が教える動物実験 第3巻 (2015)
  • Author(s): 森山 徹、弘中満太郎、藍 浩之
  • Total Pages: 4
  • Publisher: 共立出版
• [Book] コンピュータで再現！神経活動 神経細胞の電気的活動シミュレーション－、研究者が教える動物実験 第2巻 (2015)
  • Author(s): 池野英利
  • Total Pages: 5
  • Publisher: 共立出版
• [Remarks] 世界初ミツバチのダンスから蜜源への距離を読み取る脳機構に関する新発見が『J.Neurosci』に掲載
  • URL: https://wwwfukuoka-u.ac.jp/research/column/17/11/15135258.html
• [Remarks] 蜜蜂が蜜源への距離を読み取る仕組みを発見
  • URL: http://www.u-hyogo.ac.jp/shse/koho/prev/index.html
• [Remarks] Bee research may redefine understanding of ...
  • URL: https://www.japantimes.co.jp/news/2017/11/28/national/science-health/bee-research-may-redefine-understanding-intelligence/#.WwTLJkjRCUk
• [Remarks] How honeybees read the waggle dance
  • URL: https://www.sciencedaily.com/releases/2017/10/171009135435.htm
• [Remarks] How Honeybees Read the Waggle Dance
  • URL: http://neurosciencenews.com/honeybee-waggle-dance-7697/
• [Remarks] ミツバチの振動情報処理に関わる脳内神経機構に関わる日独共同研究 Ginjang
  • URL: https://projects.g-node.org/ginjang/index.html
• [Remarks] 日独共同研究＿ミツバチ言語解読の神経機構の計算機神経科学的研究チーム
  • URL: https://projects.g-node.org/ginjang/index.html