Base-excision restriction enzymes: structure, function and epigenetics
| Project/Area Number |
15K14572
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YANO Hirokazu 東京大学, 大学院新領域創成科学研究科, 特任助教 (20646773)
IDE Hiroshi 広島大学, 理学(系)研究科(研究院), 教授 (30223126)
FUKUYO Masaki 千葉大学, 大学院医学研究院, 特任助教 (40639085)
KOJIMA Kenji 東京大学, 大学院新領域創成科学研究科, 特任助教 (90748774)
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| Project Period (FY) |
2015-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 制限修飾系 / 制限酵素 / 塩基除去 / DNA切断酵素 / キャンピロバクター / ピロリ菌 / APリアーゼ / APエンドヌクレアーゼ / DNA修復 |
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| Outline of Final Research Achievements |
We earlier reported that R.PabI from hyperthermophilic arachaeon belongs to the base-excision class of restriction enzyme (DNA N-glycosylase). We purified its homologs from Campylobacterales order and demonstrated its base excision and AP lyase (DNA strand breakage) activity. They show restriction activity towards phage in Escherichia coli. For R.PabI, AP endonuclease mutants decrease the restriction.
We analyzed their molecular evolution and demonstrated they represent mobile genes. In Helicobacter pylori, the gene was lost in some East Asian strains and all Amerind strains. Recombination between East Asian strains and Indian strains took place in this locus.
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Report
(2 results)
Research Products
(13 results)
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[Presentation] 塩基切り出し型制限酵素という驚き2015
Author(s)
Masaki Fukuyo, Toshiaki Nakano, Kenji K. Kojima, Yingbiao Zhang, Yoshikazu Furuta, Ken Ishikawa, Miki Watanabe-Matsui, Hirokazu Yano, Takeshi Hamakawa, Hiroshi Ide and Ichizo Kobayashi
Organizer
日本進化学会第17回大会
Place of Presentation
中央大学後楽園キャンパス（東京都文京区）
Year and Date
2015-08-20
Related Report
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[Presentation] Methylome Diversification in Human Gastric Pathogen Helicobacter pylori2015
Author(s)
Yoshikazu Furuta, Hirokazu Yano, Zobaidul M. Alam, Emiko Rimbara, Hiroe Namba-Fukuyo, Tomoko Shibata, Tomoaki Nishiyama, Shuji Shigenobu, Mitsuyasu Hasebe, Yutaka Suzuki, Sumio Sugano, Keigo Shibayama, Ichizo Kobayashi
Organizer
International Symposium of Corrective Gene System “Establishing Next-Generation Genetics”
Place of Presentation
奈良春日野国際フォーラム 甍～I・RA・KA～（奈良県奈良市）
Year and Date
2015-05-28
Related Report
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