Development of a new protein exprassion system using LINE transposable elements

Research Project

Project/Area Number	15K14573
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Genetics/Chromosome dynamics
Research Institution	Tokyo Institute of Technology
Principal Investigator	Kajikawa masaki 東京工業大学, 生命理工学院, 講師 (90361766)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	転移因子 / レトロトランスポゾン / 遺伝子導入
Outline of Final Research Achievements	Transposable elements, called LINE, can mobilize and amplify their own copy in the host genomic DNA. Using this LINE transposable elements, we developed a novel protein expression system in cultured cells. This system will be used as a new protein expression system that can express a large amount of a certain protein you demand.