Lifespan extension by peroxidase/dual oxidase-mediated ROS signaling through pyrroloquinoline quinone in C. elegans
Project/Area Number |
15K14574
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Genetics/Chromosome dynamics
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Research Institution | Nagoya University |
Principal Investigator |
MORI Ikue 名古屋大学, 理学研究科, 教授 (90219999)
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Co-Investigator(Renkei-kenkyūsha) |
NAKANO Shunji 名古屋大学, 大学院理学研究科, 助教 (60608529)
TSUKADA Yuki 名古屋大学, 大学院理学研究科, 助教 (80580000)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 老化 / 活性酸素 / 遺伝学 / 線虫 |
Outline of Final Research Achievements |
Reactive oxygen species (ROS) are recognized as important signal molecules regulating various biological processes. Low levels of ROS released from mitochondria extend lifespan. We identified a novel mechanism of ROS-mediated lifespan extension by generating fine-tuned ROS level at the plasma membrane through a peroxidase/dual oxidase system in Caenorhabditis elegans. A redox co-factor, pyrroloquinoline quinone (PQQ), activates dual oxidase (DUOX)/BLI-3 to produce the ROS H2O2 at the plasma membrane, which is subsequently degraded by peroxidase/MLT-7 to generate the appropriate levels of ROS. The ROS signal appeared to be mediated by the oxidative stress transcriptional factors SKN-1/Nrf2 and JUN-1. We propose that low levels of ROS, fine-tuned by the peroxidase/dual oxidase system at the plasma membrane, act as second messengers, to extend lifespan by the effect of hormesis.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Reconstruction of spatial thermal gradient encoded in thermosensory neuron AFD in Caenorhabditis elegans.2016
Author(s)
Tsukada, Y., Yamao, M., Honda, N., Shimowada, T., Ohnishi, N., Kuhara, A., Ishii, S., Mori, I.
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Journal Title
J Neurosci.
Volume: 36
Issue: 9
Pages: 2571-2581
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Single-Cell memory regulates a neural circuit for sensory behavior.2016
Author(s)
Kobayashi, K., Nakano,S., Amano, M., Tsuboi, D., Nishioka, T., Ikeda, S., Yokoyama, G., Kaibuchi, K., Mori,I.
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Journal Title
Cell Rep.
Volume: 14
Issue: 1
Pages: 11-21
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] The Importance of cGMP Signaling in Sensory Cilia for Body Size Regulation in Caenorhabditis elegans.2016
Author(s)
Fujiwara, M., Hino, T., Miyamoto, R., Inada, H., Mori, I., Koga, M., Miyahara, K., Ohshima, Y., Ishihara, T.
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Journal Title
Genetics
Volume: 201
Issue: 4
Pages: 1497-1510
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A glial K+/Cl- cotransporter modifies temperature-evoked dynamics in Caenorhabditis elegans sensory neurons.2015
Author(s)
Yoshida, A., Nakano, S., Suzuki, T., Ihara, K., Higashiyama, T., Mori, I.
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Journal Title
Genes Brain Behav.
Volume: 15
Issue: 4
Pages: 429-440
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 感覚情報の時間統合を担う分子神経基盤2015
Author(s)
中野俊詩, Giles C. Andrew, 鈴木孝征, 佐野文菜, 東山哲也, 森 郁恵
Organizer
第38回日本神経科学大会
Place of Presentation
神戸国際会議場および神戸国際展示場, 神戸市
Year and Date
2015-07-28
Related Report
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[Presentation] INX-4/Innexin in AFD sensory neurons is required to regulate thermotaxis behavior in C. elegans.2015
Author(s)
Tsukamoto, S., Emmei, T.,Nishio, N., Sasakura, H., Akasaka, M. and Mori, I.
Organizer
C.elegans 20th International Meeting2015
Place of Presentation
University of California, LA, アメリカ合衆国
Year and Date
2015-06-24
Related Report
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[Presentation] The C. elegans MAST Kinase Acts through Stomatin to Regulate Thermotaxis Behavior.2015
Author(s)
Nakano,S., Giles, A. C., Ikeda, M., Suzuki, T., Sano, A., Higashiyama, T. and Mori, I.
Organizer
C.elegans 20th International Meeting2015
Place of Presentation
University of California, LA, アメリカ合衆国
Year and Date
2015-06-24
Related Report
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