| Project/Area Number |
15K14575
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Shiga University of Medical Science (2016-2018)
Kyoto University (2015) |
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Principal Investigator |
Sugi Takuma 滋賀医科大学, 神経難病研究センター, 助教 (70571305)
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| Co-Investigator(Kenkyū-buntansha) |
伊藤 浩史 九州大学, 芸術工学研究院, 准教授 (20512627)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
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| Keywords | 線虫 / ゲノム編集 / 光遺伝学 / 記憶・学習 / 行動 / ゲノム編集技術 / 遺伝子発現 / 行動遺伝学 / 生物物理 |
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| Outline of Final Research Achievements |
In this study, I established a technique, in which a gene expression of a single locus in C. elegans can be modified by genome editing technique in a cell-specific manner. I first expressed VP64 transcriptional activator in several neurons (~10 neurons) of C. elegans. This expression induces glr-1 gene in several neurons, but there were a few neurons on which VP64 has no effect. Therefore, I next tried to directly manipulate the epigenome using TALE-epigenetic factor. In this experiment, I succeeded in inducing the glr-1 expression in the neurons in which TALE-VP64 could not induce its expression. Overall, I proved that these techniques are useful to artificially induce gene expression and epigenome editing of a single locus in a cell-specific manner. Furthermore, I also insist that the techniques are also important to understand the robustness of a single locus. I am now trying to drive the gene expression and epigenome editing in a cell-specific manner by an optical manipulation.
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| Academic Significance and Societal Importance of the Research Achievements |
本手法が単純な遺伝子発現量の操作方法としてだけではなく、特定の神経細胞のクロマチン構造の「ロバストネス」を調べる方法としても有望である可能性がある. また現在, in vivoで光刺激によるエピゲノム操作を行えるように技術拡張しており、将来的に本研究により、C. elegansの記憶・学習機構を人為的に操作することにより、記憶形成に必須のエピゲノムを同定することが期待できる.
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