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Elucidation of evolutionary and molecular bases underpinning intracellular host-symbiont interactions in the seed bug Togo hemipterus

Research Project

Project/Area Number 15K14586
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Evolutionary biology
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Kikuchi Yoshitomo  国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (30571864)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords細胞内共生 / 進化 / 生物間相互作用 / 微生物 / 昆虫 / 細菌
Outline of Final Research Achievements

A number of insects possess intracellular symbiotic bacteria in specialized cells called “bacteriocytes”, wherein symbiotic bacteria are vertically transmitted. Although genomic and physiological aspects of the intracellular symbiosis have been studied, the evolutionary origin of it remains unclear. Molecular and histological investigations have strongly suggested that gut symbiosis is the origin of the intracellular symbiosis; however, the evolutionary hypothesis have never validated because of a lack of an intermediate state between gut symbiosis and highly-developed intracellular symbiosis. Recently we found that the seed bug, Togo hemipterus, which acquires its Burkholderia symbiont from environmental soil every generation, intracellularly harbors the symbiont. In this study, we have demonstrated the infection process of the symbiont, genetic bases of the symbiosis, and evolutionary diversity of the symbiotic system in heteropteran insects.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (25 results)

All 2017 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (10 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 10 results,  Open Access: 7 results,  Acknowledgement Compliant: 2 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Remarks (2 results)

  • [Int'l Joint Research] University of Bayreuth(ドイツ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] St. Petersburg SFTU(ロシア連邦)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Pusan National University(韓国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Obligate gut symbiotic association in the sloe bug Dolycoris baccarum (Hemiptera: Pentatomidae)2017

    • Author(s)
      Itoh H., Matsuura Y., Hosokawa T., Fukatsu T., Kikuchi Y.
    • Journal Title

      Applied Entomology and Zoology

      Volume: 52 Issue: 1 Pages: 51-59

    • DOI

      10.1007/s13355-016-0453-0

    • NAID

      40021101445

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Phylogenetically Diverse <i>Burkholderia</i> Associated with Midgut Crypts of Spurge Bugs, <i>Dicranocephalus</i> spp. (Heteroptera: Stenocephalidae)2016

    • Author(s)
      Stefan Martin Kuechler, 松浦優, Konrad Dettner, 菊池義智
    • Journal Title

      Microbes and Environments

      Volume: 31 Issue: 2 Pages: 145-153

    • DOI

      10.1264/jsme2.ME16042

    • NAID

      130005158505

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Collapse of insect-microbe symbiosis under simulated climate change2016

    • Author(s)
      Kikuchi Y., Tada A., Musolin D., Hari N., Hosokawa T., Fujisaki K., Fukatsu T.
    • Journal Title

      mBio

      Volume: 7 Issue: 5

    • DOI

      10.1128/mbio.01578-16

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Riptortus pedestris and Burkholderia symbiont: an ideal model system for insect-microbe symbiotic associations2016

    • Author(s)
      竹下和貴、菊池義智
    • Journal Title

      Research in Microbiology

      Volume: 168 Issue: 3 Pages: 175-187

    • DOI

      10.1016/j.resmic.2016.11.005

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Insecticide applications to soil contribute to the development of Burkholderia mediating insecticide resistance in stinkbugs2015

    • Author(s)
      Kanako Tago, Yoshitomo Kikuchi, Sinji Nakaoka, Chie Katsuyama, Masahito Hayatsu
    • Journal Title

      Molecular ecology

      Volume: 24 Issue: 14 Pages: 3766-3778

    • DOI

      10.1111/mec.13265

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ultrabithorax is essential for bacteriocyte development2015

    • Author(s)
      Yu Matsuura, Yoshitomo Kikuchi, Toru Miura, Takema Fukatsu
    • Journal Title

      Proceedings of the National Academy of Sciences of U.S.A.

      Volume: 112 Issue: 30 Pages: 9376-9381

    • DOI

      10.1073/pnas.1503371112

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bacterial cell motility ofBurkholderia gut symbiont is required to colonize the insect gut2015

    • Author(s)
      Jun Beom Lee, Jin Hee Byeon, Ho Am Jang, Jiyeun Kate Kim, Jin Wook Yoo, Yoshitomo Kikuchi, Bok Luel Lee
    • Journal Title

      FEBS letters

      Volume: 589 Issue: 19PartB Pages: 2784-2790

    • DOI

      10.1016/j.febslet.2015.08.022

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Insect’s intestinal organ for symbiont sorting2015

    • Author(s)
      Tsubasa Ohbayashi, Kazutaka Takeshita, Wataru Kitagawa, Naruo Nikoh, Ryuichi Koga, Xian-Ying Meng, Kanako Tago, Tomoyuki Hori, Masahito Hayatsu, Kozo Asano, Yoichi Kamagata, Bok Luel Lee, Takema Fukatsu, Yoshitomo Kikuchi
    • Journal Title

      Proceedings of the National Academy of Sciences of U.S.A.

      Volume: 112 Issue: 37

    • DOI

      10.1073/pnas.1511454112

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evolutionary transition in symbiotic syndromes enabled diversification of phytophagous insects on an imbalanced diet2015

    • Author(s)
      Sailendharan Sudakaran, Franziska Retz, Yoshitomo Kikuchi, Christian Kost, Martin Kaltenpoth
    • Journal Title

      The ISME journal

      Volume: 9 Issue: 12 Pages: 2587-2604

    • DOI

      10.1038/ismej.2015.75

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Burkholderia of Plant-Beneficial Group are Symbiotically Associated with Bordered Plant Bugs (Heteroptera: Pyrrhocoroidea: Largidae)2015

    • Author(s)
      Kazutaka Takeshita, Yu Matsuura, Hideomi Itoh, Ronald Navarro, Tomoyuki Hori, Teruo Sone, Yoichi Kamagata, Peter Mergaert, Yoshitomo Kikuchi
    • Journal Title

      Microbes and Environments

      Volume: 30 Issue: 4 Pages: 321-329

    • DOI

      10.1264/jsme2.me15153

    • NAID

      130005116033

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] コバネヒョウタンナガカメムシにおける環境獲得型の細胞内共生の発見2017

    • Author(s)
      竹下和貴、松浦優、菊池義智
    • Organizer
      日本応用動物昆虫学会・日本昆虫学会2016年度北海道支部会
    • Place of Presentation
      北海道大学博物館(北海道札幌市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒョウタンナガカメムシ類で見つかった環境獲得型細胞内共生2017

    • Author(s)
      竹下和貴、松浦優、孟憲英、三谷恭雄、新里尚也、菊池義智
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      東京農工大学(東京都小金井市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Evolutionary innovation through symbiotic association with microorganisms2016

    • Author(s)
      菊池義智
    • Organizer
      日本昆虫学会・日本応用動物昆虫学会合同大会
    • Place of Presentation
      大阪府立大学(大阪府堺市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Mutualistic endosymbiosis in insects: its diversity and molecular basis2016

    • Author(s)
      菊池義智
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター(大阪府大阪市)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Gut Symbiotic Bacteria in Stink Bugs Confer Pesticide Resistance2016

    • Author(s)
      菊池義智
    • Organizer
      ASM Microbe 2016
    • Place of Presentation
      Boston Convention and Exhibition Center(アメリカ合衆国、マサチューセッツ州ボストン)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] カメムシとBurkholderia細菌を結ぶ共生戦略とは?2016

    • Author(s)
      大林翼、菊池義智
    • Organizer
      日本進化学会第18回東京大会
    • Place of Presentation
      東京工業大学(東京都大岡山)
    • Related Report
      2016 Annual Research Report
  • [Presentation] カメムシ類の腸内共生細菌に関する研究~垂直伝播を伴わない昆虫内部共生系の発見、そしてその発展~2016

    • Author(s)
      菊池義智
    • Organizer
      日本微生物生態学会第31回大会
    • Place of Presentation
      横須賀市文化会館(神奈川県横須賀市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 環境から獲得される細胞内共生細菌:ナガカメムシ類で見つかった腸内共生から細胞内共生へのミッシングリンク2016

    • Author(s)
      竹下和貴、松浦優、孟憲英、三谷恭雄、菊池義智
    • Organizer
      日本微生物生態学会第31回大会
    • Place of Presentation
      横須賀市文化会館(神奈川県横須賀市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 共生微生物による昆虫の農薬抵抗性進化2015

    • Author(s)
      菊池義智
    • Organizer
      菌根研究会2015年大会
    • Place of Presentation
      とかちプラザ(北海道帯広市)
    • Year and Date
      2015-10-03
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] カメムシに殺虫剤抵抗性を賦与するBurkholderia共生細菌2015

    • Author(s)
      菊池義智
    • Organizer
      日本生物地理学会第70回年次大会
    • Place of Presentation
      立教大学(東京都豊島区)
    • Year and Date
      2015-04-12
    • Related Report
      2015 Research-status Report
    • Invited
  • [Remarks] 菊池チーム

    • URL

      https://staff.aist.go.jp/y-kikuchi/index.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 菊池研究室

    • URL

      https://staff.aist.go.jp/y-kikuchi/index.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2022-02-16  

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