| Project/Area Number |
15K14623
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KAZAMA TOMOHIKO 東北大学, 大学院農学研究科, 助教 (30431464)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 育種学 / 遺伝学 / 遺伝子 / バイオテクノロジー / 植物 |
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| Outline of Final Research Achievements |
To establish genome-editing system in rice mitochondria, we developed a rice cell suspension lines with enlarged mitochondria mediated by dominant-negative dynamin-like transgene. We introduced a GFP gene using particle gun. We did not, however, detect introduced GFP expression in the mitochondria. We constructed a mini-circle DNA containing a GFP expression cassette and plasmid-like B1 DNA, which was stably present in mitochondria of certain cultivars. The resulting vector can be used as a basic tool for genome-editing experiments in rice mitochondria.
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