Exploring new regulatory layers for embryo patterning by a proteome approach in rice

• Project/Area Number: 15K14625
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: National Institute of Genetics (2016-2017); Nagoya University (2015)
• Principal Investigator: SATO Yutaka 国立遺伝学研究所, 系統生物研究センター, 教授 (40345872)
• Co-Investigator(Renkei-kenkyūsha): TADA Yasuomi 名古屋大学, 遺伝子実験施設, 教授 (40552740) ; MORI Hitoshi 名古屋大学, 大学院・生命農学研究科, 教授 (20220014)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: イネ / プロテオーム / 質量分析 / ペプチド

Outline of Final Research Achievements

So far, abundant proteins or easily ionized proteins are dominantly detected by the proteome analysis. This makes sometimes difficult to detect rare proteins or proteins with difficulties in ionization. This could often be a big obstacle to analyze developmental processes by proteomic analysis because developmental processes are usually regulated by small amount of signaling molecules or proteins. I am engaged in rice developmental biology for a long time. Using this background, I conducted to set up condition to apply proteomic approach to rice developmental approach.

Research Products

• [Journal Article] Genetic analysis of rice mutants responsible for narrow leaf phenotype and reduced vein number (2016)
  • Author(s): Kubo, F.C., Yasui, Y., Kumamaru, T., Sato, Y., and Hirano, H.-Y.
  • Journal Title: Genes & Genetic Systems Volume: 91 Issue: 4 Pages: 235-240
  • DOI: 10.1266/ggs.16-00018
  • NAID: 130005450049
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Book] ノンコーディングRNA (2016)
  • Author(s): 都筑正行、濱田隆宏、佐藤 豊
  • Total Pages: 372
  • Publisher: 化学同人