Synthesis of a specific RNA-binding protein via the artificial evolution of Rf-PPR homologs

Research Project

Project/Area Number	15K14626
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Science in genetics and breeding
Research Institution	The University of Tokyo (2017) Nara Institute of Science and Technology (2015-2016)
Principal Investigator	Fujii Sota 東京大学, 大学院農学生命科学研究科(農学部), 助教 (90716713)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	稔性回復遺伝子 / 人工進化 / RNA編集
Outline of Final Research Achievements	In the age of genome-editing, technologies that enable engineering of RNA expression is demanded. In this project, I aimed to establish such system by using the Rf-PPR protein, an RNA-binding protein with great diversity and ubiquitously present in angiosperm species. First of all, I established a system that visualizes the binding of a protein to an RNA sequence in E. coli. I have also performed functional characterization of an Rf-PPR homolog. The RFL2 was shown to be involved in the cleavage of the RNA transcript of the mitochondrially-encoded orf291.

Report

(4 results)