Synthesis of a specific RNA-binding protein via the artificial evolution of Rf-PPR homologs
Project/Area Number |
15K14626
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Science in genetics and breeding
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Research Institution | The University of Tokyo (2017) Nara Institute of Science and Technology (2015-2016) |
Principal Investigator |
Fujii Sota 東京大学, 大学院農学生命科学研究科(農学部), 助教 (90716713)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 稔性回復遺伝子 / 人工進化 / RNA編集 |
Outline of Final Research Achievements |
In the age of genome-editing, technologies that enable engineering of RNA expression is demanded. In this project, I aimed to establish such system by using the Rf-PPR protein, an RNA-binding protein with great diversity and ubiquitously present in angiosperm species. First of all, I established a system that visualizes the binding of a protein to an RNA sequence in E. coli. I have also performed functional characterization of an Rf-PPR homolog. The RFL2 was shown to be involved in the cleavage of the RNA transcript of the mitochondrially-encoded orf291.
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Report
(4 results)
Research Products
(2 results)
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[Journal Article] Calcium signalling mediates self-incompatibility response in the Brassicaceae2015
Author(s)
Megumi Iwano, Kanae Ito, Sota Fujii, Mitsuru Kakita, Hiroko Asano-Shimosato, Motoko Igarashi, Pulla Kaothien-Nakayama, Tetsuyuki Entani, Asaka Kanatani, Masashi Takehisa, Masaki Tanaka, Kunihiko Komatsu, Hiroshi Shiba, Takeharu Nagai, Atsushi Miyawaki, Akira Isogai & Seiji Takayama
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Journal Title
Nature Plants
Volume: 1
Issue: 9
Pages: 15128-15128
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research