• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on the replication mechanism of a mini circle found in transplastomic plants and a vector development

Research Project

Project/Area Number 15K14629
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Science in genetics and breeding
Research InstitutionKyoto Sangyo University

Principal Investigator

TERACHI Toru  京都産業大学, 総合生命科学部, 教授 (90202192)

Co-Investigator(Kenkyū-buntansha) 木村 成介  京都産業大学, 総合生命科学部, 教授 (40339122)
山岸 博  京都産業大学, 総合生命科学部, 教授 (10210345)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords葉緑体 / 形質転換 / タバコ / ミニサークル / 斑入り / apx / プラスミド / 遺伝子組換え / DNA複製 / 葉緑体形質転換 / 葉緑体ゲノム / プラスミドベクター / パーティクルガン / 複製機構
Outline of Final Research Achievements

In order to clarify the replication mechanism of a “mini circle” found in the chloroplast of transplastomic tobacco plants, plasmids containing a different chloroplast DNA fragment from the mini circle and the antibiotic resistant gene aadA were constructed. Then, the plasmids were introduced into tobacco leaves by particle bombardment experiments. All of three plasmids constructed with a different chloroplast DNA fragment produced antibiotic resistant shoots, showing that these plasmids could replicate in the chloroplast. Deletion mutants from the plasmid #2A also yielded antibiotic resistant shoots, indicating a part of the #2A fragment contained replication origin that were functional in the chloroplast.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2018 2017 2016 2015

All Presentation (8 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] 部分二倍体となった葉緑体ゲノムをもつ形質転換タバコの性状2018

    • Author(s)
      植村香織、寺地徹
    • Organizer
      日本育種学会第133回講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 葉緑体内で自律複製する新たな形質転換ベクターの開発2017

    • Author(s)
      植村香織、児島和志、寺地徹
    • Organizer
      日本育種学会第131回講演会
    • Place of Presentation
      名古屋大学東山キャンパス(愛知県・名古屋市)
    • Year and Date
      2017-03-30
    • Related Report
      2016 Research-status Report
  • [Presentation] Development of new transformation vectors that are autonomously replicable in the organelle2017

    • Author(s)
      Kaori Uemura and Toru Terachi
    • Organizer
      10th International Conference for Plant Mitochondrial Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物の葉緑体ゲノムを分割する方法の開発2017

    • Author(s)
      植村香織、児島和志、寺地徹
    • Organizer
      日本育種学会第132回講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 自律複製する葉緑体形質転換ベクターの複製起点に関する研究2017

    • Author(s)
      児島和志、植村香織、寺地徹
    • Organizer
      日本育種学会第132回講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 葉緑体の遺伝子組換えタバコ作出の過程で得られた斑入り系統の解析 IV. ミニサークルを利用した新規葉緑体形質転換ベクターの構築2016

    • Author(s)
      植村香織、林未来、寺地徹
    • Organizer
      日本育種学会第130回講演会
    • Place of Presentation
      鳥取大学農学部(鳥取県・鳥取市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Presentation] ダイズの鉄貯蔵タンパクferritinを葉緑体ゲノムに導入した形質転換レタスの特徴づけ2016

    • Author(s)
      阿部こころ、井上理恵子、植村香織、寺地徹
    • Organizer
      日本育種学会第130回講演会
    • Place of Presentation
      鳥取大学農学部(鳥取県・鳥取市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Presentation] 葉緑体の遺伝子組換えタバコ作出の過程で得られた斑入り系統の解析 III. マルチパータイト構造をとる葉緑体ゲノム2015

    • Author(s)
      植村 香織, 森田 重人, 山本 真紀, 高見 常明, 坂本 亘, 寺地 徹
    • Organizer
      日本育種学会 第128回講演会
    • Place of Presentation
      新潟大学(新潟県・新潟市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi