Characterization of dehydration-inducible icl gene in rice

• Project/Area Number: 15K14631
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: Japan International Research Center for Agricultural Sciences
• Principal Investigator: Maruyama Kyonoshin 国立研究開発法人国際農林水産業研究センター, 生物資源・利用領域, 主任研究員 (10425530)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: icl / ABA / rice / sugar / 糖代謝 / メタボローム / トランスクリプトーム

Outline of Final Research Achievements

In this study, we investigated the functions of isocitrate lyase (icl) gene of rice using deficient mutants and overexpressors. Transcripts analysis indicated that icl gene expression was regulated by ABA under dehydration conditions. Metabolites analysis indicated that icl was involved in sugar metabolism. Our integrated analyses suggest that ICL is a key enzyme in sugar metabolism under dehydration conditions.