Research Project
Grant-in-Aid for Challenging Exploratory Research
Agrobacterium-mediated transformation is a gold-standard for gene integration in to rice genome. This project aimed to establish the methodology for cell type specific epigenome analysis and to analyze transcriptome dynamics during Agrobacterium infection. We successfully applied INTACT method to rice to isolate pure nuclei from cells of interest. Time-series RNA-seq revealed Agrobacterium infection induced expression of mitochondria related genes only after 3 days after infection. These results provide the insights for rice callus responses to Agrobacterium infection to improve transformation efficiency.
All 2016
All Presentation (1 results) (of which Invited: 1 results)