Epigenomic change induced by Agrobacterium infection

• Project/Area Number: 15K14632
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: National Agriculture and Food Research Organization (2016-2017); 国立研究開発法人農業生物資源研究所 (2015)
• Principal Investigator: Kawakatsu Taiji 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 主任研究員 (30435614)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: イネ / アグロバクテリウム / 形質転換 / カルス / エピゲノム

Outline of Final Research Achievements

Agrobacterium-mediated transformation is a gold-standard for gene integration in to rice genome. This project aimed to establish the methodology for cell type specific epigenome analysis and to analyze transcriptome dynamics during Agrobacterium infection. We successfully applied INTACT method to rice to isolate pure nuclei from cells of interest. Time-series RNA-seq revealed Agrobacterium infection induced expression of mitochondria related genes only after 3 days after infection. These results provide the insights for rice callus responses to Agrobacterium infection to improve transformation efficiency.

Research Products

• [Presentation] エピゲノム情報から読み解く細胞アイデンティティー (2016)
  • Author(s): 川勝泰二
  • Organizer: 第34回日本植物細胞分子生物学会講演会 シンポジウム
  • Place of Presentation: 信州大学（長野県・上田市）
  • Invited