Understanding of the pre-adaptation mechanisms generated by cryptic genetic variation

• Project/Area Number: 15K14634
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Onda Yoshihiko 国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (50547073)
• Research Collaborator: MOCHIDA Keiichi 国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (90387960) ; SHIMIZU Minami 国立研究開発法人理化学研究所, 環境資源科学研究センター, テクニカルスタッフ ; UEHARA Yukiko 国立研究開発法人理化学研究所, 環境資源科学研究センター, テクニカルスタッフ ; KATO Fumiko 国立研究開発法人理化学研究所, 環境資源科学研究センター, パートタイマー ; NAKAYAMA Risa 国立研究開発法人理化学研究所, 環境資源科学研究センター, パートタイマー
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ミナトカモジグサ / Brachypodium / 高温耐性 / QTL / SNP

Outline of Final Research Achievements

Brachypodium distachyon is an annual grass species that belongs to the Pooideae subfamily and is phylogenetically related to economically important temperate cereals such as wheat and barley. We performed heat stress test with several natural accessions, and found the difference in heat stress tolerance among natural accessions. We found single QTL for heat stress tolerance on chromosome 4 by QTL analysis with F2 mapping population. Moreover, we could narrow the QTL down to 220 kb containing 40 annotated genes by using F3 mapping population.

Research Products

• [Journal Article] Exploring Genetic Diversity in Plants by Using High-Throughput Sequencing Techniques. (2016)
  • Author(s): Onda, Y. and Mochida, K.
  • Journal Title: Current Genomics Volume: 17 Pages: 356-365
  • Peer Reviewed / Open Access
• [Journal Article] Expression profiling of marker genes responsive to the defence-associated phytohormones salicylic acid, jasmonic acid and ethylene in Brachypodium distachyon. (2016)
  • Author(s): Kouzai, Y., Kimura, M., Yamanaka, Y., Watanabe, M., Matsui, H., Yamamoto, M., Ichinose, Y., Toyoda, K., Onda, Y., Mochida, K. and Noutoshi, Y.
  • Journal Title: BMC Plant Biology Volume: 16 Issue: 1 Pages: 1-11
  • DOI: 10.1186/s12870-016-0749-9
  • Peer Reviewed / Open Access
• [Journal Article] Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops. (2015)
  • Author(s): Onda, Y., Hashimoto, K., Yoshida, T., Sakurai, T., Sawada, Y., Hirai, M.Y., Toyooka, K., Mochida, K. and Shinozaki, K.
  • Journal Title: Proceedings of the Royal Society B Volume: 282 Issue: 1811 Pages: 1-8
  • DOI: 10.1098/rspb.2015.0964
  • Peer Reviewed / Open Access
• [Journal Article] 新しいモデル草本植物 ミナトカモジグサ (Brachypodium distachyon) (2015)
  • Author(s): 持田恵一, 恩田義彦, 佐々木忠将, 氷室泰代, 最相大輔, 小林正智, 平山隆志, 篠崎一雄
  • Journal Title: 植物の生長調節 Volume: 50 Pages: 103-109
  • Peer Reviewed
• [Presentation] A simple and versatile SNP genotyping method by multiplex PCR amplicon sequence in Brachypodium distachyon. (2017)
  • Author(s): Yoshihiko Onda, Minami Shimizu, Kotaro Takahagi, Keiichi Mochida
  • Organizer: International Plant & Animal Genome XXV
  • Place of Presentation: San Diego, USA
  • Year and Date: 2017-01-14
  • Int'l Joint Research
• [Presentation] 発熱植物における「花の匂い生産」についてのトランスクリプトーム解析 (2016)
  • Author(s): 恩田義彦, 持田恵一, 吉田拓広, 櫻井哲也, Roger Seymour, 梅川結, Stergios Pirintsos, 篠崎一雄, 伊藤菊一.
  • Organizer: 第63回日本生態学会仙台大会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2016-03-20
• [Presentation] Artificial crossing method and development of population genotyping technique based on amplicon sequence in Brachypodium distachyon. (2016)
  • Author(s): Yoshihiko Onda, Minami Shimizu, Kotaro Takahagi, Fumiko Kato, Risa Nakayama, Keiichi Mochida.
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] Introduction to genome duplication and the allopolyploid Arabidopsis kamchatica and Cardamine spp. as model polyploid species to study ecological speciation. (2015)
  • Author(s): Kentaro Shimizu, Tim Paape, Masaomi Hatakeyama, Reiko Akiyama, Rie Shimizu-Inatsugi, Satoru Akama, Jun Sese, Tanaka Kenta, Yoshihiko Onda.
  • Organizer: 日本進化学会第17回大会
  • Place of Presentation: 中央大学後楽園キャンパス
  • Year and Date: 2015-08-20
• [Remarks] ミナトカモジグサとコムギの蓄積代謝物の共通点と相違点を解明
  • URL: http://www.riken.jp/pr/press/2015/20150715_1/
• [Remarks] 「ミナトカモジグサとコムギの蓄積代謝物の共通点と相違点」
  • URL: http://www.riken.jp/pr/blog/2015/150824_1/