| Project/Area Number |
15K14642
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Crop production science
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| Research Institution | Tokuyama College of Technology |
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Principal Investigator |
ISHII Takaaki 徳山工業高等専門学校, その他部局等, 研究員 (30136296)
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| Co-Investigator(Kenkyū-buntansha) |
天内 和人 徳山工業高等専門学校, 一般科目, 教授 (20390502)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 菌根菌 / 菌根共生タンパク質 / 菌根菌検査薬 / 携帯型蛍光顕微鏡装置 / 新開発の携帯型蛍光検出器 |
|---|
| Outline of Final Research Achievements |
Mycorrhizal fungi, particularly arbuscular mycorrhizal fungi (AMF) that infect almost all of the plants, play a very important role for the maintenance of natural ecology and the construction of safe, secure and sustainable crop production. The standard procedure to observe root colonization, however, is cumbersome, and various light microscopical techniques are required to clearly distinguish between AMF and root tissues. Recently, the fluorescein-labeled specific antibody against 24 kDa protein related to mycorrhizal symbiosis was developed. The new antibody was successfully used to detect various kinds of mycorrhizal fungi in fluorescence microscopic observation. In this study, we developed new technique for observing mycorrhizal fungi easily by using a newly developed portable fluorescence microscope with the detection reagent.
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