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Application of NMR-based metabolomics to the development of marker-assisted breeding and new cultivation technique

Research Project

Project/Area Number 15K14643
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Crop production science
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

SEKIYAMA Yasuyo  国立研究開発法人農業・食品産業技術総合研究機構, 食品研究部門, 上級研究員 (60342804)

Co-Investigator(Kenkyū-buntansha) 岡崎 和之  国立研究開発法人農業・食品産業技術総合研究機構, 北海道農業研究センター, 主任研究員 (90549239)
Co-Investigator(Renkei-kenkyūsha) IKEDA Seishi  国立研究開発法人農業・食品産業技術総合研究機構, 北海道農業研究センター, 上級研究員 (20396609)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsNMR / メタボロミクス / ジャガイモ / テンサイ / そうか病 / 褐斑病 / 代謝マーカー / 育種・栽培技術 / NMR
Outline of Final Research Achievements

A better understanding of the plant metabolism response to environmental stresses, including pathogen infections, can help us understand plant physiology, which is crucial for the future applications of plant metabolomics in plant breeding and development of new cultivation techniques. In this study, we report the NMR-based metabolic profiling of field-grown leaves for a subset of potato and sugar beet genotypes, which are the major field crops in Japan.
Common scab is one of the most serious soil-borne diseases of potato tubers in the world. At first, we clarified that ratio of foliar citrate to formate could be a marker for differentiating between normal and artificial field infested with common scab.
Cercospora leaf spot (CLS), which is caused by the fungus Cercospora beticola Sacc., is one of the most serious leaf diseases for sugar beet (Beta vulgaris L.) worldwide. In our sugar beet field trial, GABA and Gln roughly differentiate susceptible genotypes from resistant genotypes.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Proposal for Practical Application of Field Metabolomics2017

    • Author(s)
      関山恭代、池田成志、冨田理
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 55 Issue: 6 Pages: 392-399

    • DOI

      10.1271/kagakutoseibutsu.55.392

    • NAID

      130006742954

    • ISSN
      0453-073X, 1883-6852
    • Year and Date
      2017-05-20
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NMR-Based Metabolic Profiling of Field-Grown Leaves from Sugar Beet Plants Harbouring Different Levels of Resistance to Cercospora Leaf Spot Disease2017

    • Author(s)
      Yasuyo Sekiyama, Kazuyuki Okazaki, Seishi Ikeda, Jun Kikushi
    • Journal Title

      Metabolites

      Volume: 7 Issue: 1 Pages: 4-4

    • DOI

      10.3390/metabo7010004

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 農業・食品産業現場におけるNMRメタボロミクスの利用と今後の展望2015

    • Author(s)
      関山恭代、池田成志、冨田理
    • Journal Title

      バイオインダストリー (シーエムシー出版)

      Volume: 379 Pages: 10-16

    • Related Report
      2015 Research-status Report
    • Acknowledgement Compliant
  • [Presentation] 農業・食品産業現場でのメタボロミクスの活用に向けて2017

    • Author(s)
      関山恭代
    • Organizer
      次世代農業最前線セミナー
    • Place of Presentation
      とかちプラザ(北海道帯広市)
    • Year and Date
      2017-03-25
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 褐斑病抵抗性の異なるテンサイのメタボローム解析2017

    • Author(s)
      関山恭代、岡崎和之、池田成志、菊地 淳
    • Organizer
      第16回 産総研・産技連LS-BT合同研究発表会
    • Place of Presentation
      産業技術総合研究所(茨城県つくば市)
    • Year and Date
      2017-01-31
    • Related Report
      2016 Research-status Report
  • [Presentation] NMRデータから見る農と食のサイエンス2017

    • Author(s)
      関山恭代
    • Organizer
      JASIS2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 農業・食品分野における微生物科学とメタボロミクスの連携の可能性について2017

    • Author(s)
      池田成志、関山恭代
    • Organizer
      第11回メタボロームシンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] メタボロミクスによる作物・農産物の成分多様性に影響する環境要因の評価と農学・食品科学のパラダイムシフト2016

    • Author(s)
      関山恭代
    • Organizer
      自然共生型農業研究シンポジウム2016
    • Place of Presentation
      とかちプラザレインボーホール(北海道帯広市)
    • Year and Date
      2016-11-22
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] NMR-based metabolic profiling of field-grown leaves from sugar beet plants harbouring different resistance levels to Cercospora leaf spot disease2016

    • Author(s)
      Yasuyo Sekiyama, Kazuyuki Okazaki, Seishi Ikeda, Jun Kikushi
    • Organizer
      12th International Conference of the Metabolomics Society
    • Place of Presentation
      ダブリン(アイルランド)
    • Year and Date
      2016-06-27
    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 農業・食品研究におけるNMRメタボロミクスの利用2016

    • Author(s)
      関山恭代
    • Organizer
      農業食料工学会 食料・食品工学部会シンポジウム
    • Place of Presentation
      東京都立食品技術センター(東京都千代田区)
    • Year and Date
      2016-02-22
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] NMR-based metabolic profiling of potato leaves for the identification of potential metabolic indicators of common scab resistance in potato tubers2015

    • Author(s)
      Satoru Tomita, Kenji Asano, Seishi Ikeda, Jun Kikuchi, Eisuke Chikayama, Hiroshi Ono, Akira Kobayashi, Yuki O. Kobayashi, Yasuyo Sekiyama
    • Organizer
      11th International Conference of the Metabolomics Society
    • Place of Presentation
      サンフランシスコ(アメリカ)
    • Year and Date
      2015-06-29
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] ジャガイモ作物体の生育状態診断方法2015

    • Inventor(s)
      関山恭代、冨田理、小野裕嗣、池田成志、浅野賢治、小林晃、小林有紀
    • Industrial Property Rights Holder
      関山恭代、冨田理、小野裕嗣、池田成志、浅野賢治、小林晃、小林有紀
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-05-29
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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