| Project/Area Number |
15K14646
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Horticultural science
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 自家不和合性 / サクラ属果樹 / 花粉形質転換 / パーティクルボンバードメント |
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| Outline of Final Research Achievements |
In this study, the self incompatibility (SI)-assisted pollen-mediated transformation approach was proposed and tested for the alternative transformation technique for Prunus fruit trees. At first, the hairpin gene construct for silencing the pollen SI determinant gene was designed, and its silencing ability was confirmed by the assay using the genetic transformed tobacco plants. Next, the transient-expression vector was developed which contained the hairpin gene and GUS marker gene under control of the pollen-specific LAT52 promoter. The transformation efficiency of sweet cherry ‘Satonishiki’ pollen by particle bombardment was up to 1 % at the optimized condition. Finally, 500~ flowers on ‘Satonishiki’ potted trees were self-pollinated with bombarded-pollen, but no fruit was set. The further study testing the efficiency of the proposed approach was needed to make a conclusion.
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