| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In order to develop DNA markers for identification of intra-cultivars and for discrimination of production region, ion-beam-irradiation was performed for Citrus seedlings except roots. Seedlings obtained from 0 - 30 Gy treatments showed normal growth similar to thier controls. However, 50 Gy-irradiated seedlings showed dwarf growth, variegation and mutations of leaf shape. DNA polymorphism was detected from one of them. After grafting propagation, all clones stably maintained the same of DNA polymorphism.
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