Project/Area Number |
15K14655
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Horticultural science
|
Research Institution | Ehime University |
Principal Investigator |
HABU Tsuyoshi 愛媛大学, 農学研究科, 准教授 (60335304)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | カンキツ / 裂果 / RNA-seq / 気孔 |
Outline of Final Research Achievements |
In this study, to elucidate the mechanism of fruit splitting, we compared morphological and physiological characteristics and gene expressions between fruit splitting sensitive and insensitive citrus cultivars. Numbers of stomata were slight different, but fruit weight increase by water absorption from the peel surface were not different between cultivars, suggesting that fruit splitting in citrus is not likely due to water absorption from the peel surface. On the other hand, juice sac weight rapidly increased during the autumn rainy season, in which fruit splitting occurred the most. We supposed that a combination of a thin peel and this rapid enlargement of juice sacs is probably related to fruit splitting. RNA-seq analyses showed that genes related to cell enlargement differently expressed between cultivars. These different expression patterns may be related to differences of the juice sac enlargement, which could lead fruit splitting.
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