Studies on practical application of plant peptide hormones in horticultural science

• Project/Area Number: 15K14656
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Horticultural science
• Research Institution: University of Miyazaki
• Principal Investigator: Tetsumura Takuya 宮崎大学, 農学部, 教授 (00227498)
• Co-Investigator(Kenkyū-buntansha): 冨永 るみ 広島大学, 生物圏科学研究科, 准教授 (20373334) ; 澤 進一郎 熊本大学, 大学院先端科学研究部(理), 教授 (00315748)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 果樹 / ペプチドホルモン / メリステム / 植物組織培養 / 園芸科学

Outline of Final Research Achievements

CLE25, a member of CLE peptide family, was added to the root-inducing medium and found to inhibit growth of ‘Hiratanenashi’ persimmon root. The treatment after the formation of root primordia was more effective than that at the start of the root induction. Microscopic observations revealed that the cell division of the cortical tissue in the treated roots was inhibited so that the roots were thinner. The root-inducing medium with CLE25 inhibited elongation of roots of ‘Pino Noir’ grape, whereas the roots did not become thin. CLE25 treatments to shoot apical meristems of ‘Hiratanenashi’ and ‘Pino Noir’ had no effect on their growth. As for CLE14, another plant peptide hormone, the treatment to Arabidopsis seedlings reduced in primary root length and induced excess root hair formation.

Research Products

• [Journal Article] CLE14 peptide signaling in Arabidopsis root hair cell fate determination (2018)
  • Author(s): Naoto Hayashi, Takuya Tetsumura, Shinichiro Sawa, Takuji Wada, and Rumi Tominaga-Wada
  • Journal Title: Plant Biotechnology Volume: 35 Issue: 1 Pages: 17-22
  • DOI: 10.5511/plantbiotechnology.18.0122a
  • NAID: 130006727675
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Open Access
• [Journal Article] Effects of CLE peptides on growth of in vitro roots and shoots of persimmon (2018)
  • Author(s): T. Tetsumura, Y. Osako, S. Ishimura, C. Honsho, S. Sawa, T. Wada, R. Tominaga
  • Journal Title: Acta Horticulturae Volume: 1195 Issue: 1195 Pages: 93-97
  • DOI: 10.17660/actahortic.2018.1195.16
  • Peer Reviewed
• [Presentation] ペプチドホルモンCLE14がシロイヌナズナの根毛形成機構に及ぼす影響 (2018)
  • Author(s): 林 直人・鉄村琢哉・澤進一郎・和田拓治・冨永るみ
  • Organizer: 日本農芸化学会中四国支部第50回記念講演会
• [Presentation] Effects of CLE peptides on growth of in vitro roots and shoots of persimmon (2016)
  • Author(s): Tetsumura, T., Osako, Y., Ishimura, S., Honsho, C., Sawa, S., Wada, T., Tominaga, R.
  • Organizer: VI International Symposium on Persimmon
  • Place of Presentation: ADEIT, Valencia, Spain
  • Year and Date: 2016-10-16
  • Int'l Joint Research
• [Presentation] SOL1 and other peptidases are responsible for CLE peptide processing mechanisms (2016)
  • Author(s): Shinichiro Sawa
  • Organizer: 22nd International conference on plant growth substances
  • Place of Presentation: Victoria College, Toronto, Canada
  • Year and Date: 2016-06-21
  • Int'l Joint Research