Project/Area Number |
15K14658
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Horticultural science
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Research Institution | Iwate Biotechnology Research Center |
Principal Investigator |
Takahashi Hideyuki 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (00455247)
|
Research Collaborator |
FUJITA KOUHEI 公益財団法人 岩手生物工学研究センター, 園芸資源研究部, 研究助手
YOSHIDA CHIHARU 公益財団法人 岩手生物工学研究センター, 園芸資源研究部, 研究助手
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | 花卉 / 休眠 |
Outline of Final Research Achievements |
Dormancy caused by nutritional starvation is a specific regulatory system of bacteria and fungi. This dormancy is mediated by stringent response that is regulated by RelA/SpoT Homologs (RSH). However, we previously found that gentian plantlets cultured under P deficient condition produced dormant organ, indicating the possibility that plants also possess a dormancy mechanism regulated by stringent response. In this study, we identified two RSH genes (RSH1 and CRSH) and found that the expression level of these genes increased by P starvation in gentian plantlets. Furthermore, we identified 13 variants of RSH1 using a next generation sequence technique. Interestingly, the expression ratio of functional variant was higher in plantlets cultured under P deficient condition. The ratio was also higher in dormant organ of field grown gentian plants. These results imply that P concentration-dependent regulation of RSH may be involved in dormancy mechanism in gentian.
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