Exploration of infection mechanism and utilization of a mitochondrially replicating virus

• Project/Area Number: 15K14663
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: Okayama University
• Principal Investigator: SUZUKI Nobuhiro 岡山大学, 資源植物科学研究所, 教授 (70206514)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ミトコンドリア / ミトウイルス / 菌類ウイルス

Outline of Final Research Achievements

Mitoviruses are a unique group of mitochondrially replicating viruses with positive-sense, single stranded RNA genomes. Among them is Cryphonectria mitovirus 1 (CpMV1) which infects one of the three most destructive tree pathogens, chestnut blight fungus. This pathosystem involves two host/parasite interactions (chestnut vs. chestnut blight fungus) and chestnut blight fungus vs. CpMV1). This study represents the first example in which the host range, lateral transmissibility, symptom expression and counter-defense mechanism of a mitovirus was thoroughly analyzed.

Research Products

• [Int'l Joint Research] Rutgers University(米国)
• [Journal Article] Viruses of plant-interacting fungi. (2018)
  • Author(s): Hillman, B. I., Aulia, A., and Suzuki, N.
  • Journal Title: Advances in Virus Research Volume: 100 Pages: 99-116
  • DOI: 10.1016/bs.aivir.2017.10.003
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Integration of viral sequences into eukaryotic host genomes: legacy of ancient infections (2018)
  • Author(s): Tomonaga, K., Berkhout, B., and Suzuki, N.
  • Journal Title: Virus Research Volume: in press
  • Int'l Joint Research
• [Presentation] Host expansion of Cryphonectria mitovirus 1 revealed its avoidance of antiviral RNA silencing (2018)
  • Author(s): Shahi, S., Eusebio-Cope, A., Chiba, S., Hillman, B. I., and Suzuki, N.
  • Organizer: The Annual Meeting of the Japanese Phytopathological Society
• [Presentation] Avoidance of antiviral RNA silencing by Cryphonectria mitovirus 1 revealed by the expansion of its hosts. (2017)
  • Author(s): Shahi, S., Eusebio-Cope, A., Chiba, S., Hillman, B. I., Nobuhiro Suzuki, N.
  • Organizer: 4th International Mycovirus Symposium.
  • Int'l Joint Research
• [Presentation] Development of molecular and biologic tools for studying a mitovirus from Cryphonectria parasitica. (2016)
  • Author(s): Shahi, S., Hyodo, K., Chiba. S., Hillman. B. I., and Suzuki, N.
  • Organizer: The Annual Meeting of the Kansai Regional Branch of Japanese Phytopathological Society.
  • Place of Presentation: Shizuoka
  • Year and Date: 2016-09-29
• [Remarks]
  • URL: http://www.rib.okayama-u.ac.jp/pmi/publication.html
• [Remarks] 植物・微生物相互作用グループ業績
  • URL: http://www.rib.okayama-u.ac.jp/pmi/publication.html