Development of a system to confer plant virus disease tolerance through the regulation of reactive oxygen species production in chloroplasts
| Project/Area Number |
15K14664
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Ehime University |
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Principal Investigator |
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| Research Collaborator |
KOSAKA Naomi
YAMASHITA Mei
OKAMOTO Maiko
BHOR Sachin Ashok
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 植物ウイルス病 / 遺伝子組換え植物 / 活性酸素種 / 葉緑体 / 植物病害 / タンパク質輸送 / 非翻訳領域 / 植物 / 遺伝子組換え / 病害応答 |
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| Outline of Final Research Achievements |
The present study aimed to generate plants tolerant to plant virus diseases. To this end, we designed a genetically modified plant system to express timely the gene(s) regulating the reactive oxygen species (ROS) production in chloroplasts, which is known to have roles in plant disease development. We established a cheap method to introduce foreign genes into chloroplast and introduced genes that can regulate ROS production into tobacco chloroplasts. Through the examination of a switching gene that turns on the foreign gene in chloroplasts, we found that both protein and RNA structures are important to enable the switching gene product for its efficient transport into chloroplasts.
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Report
(4 results)
Research Products
(16 results)
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[Presentation] Artificial induction of chlorosis symptom through the silencing of chloroplast genes in transgenic tobacco2016
Author(s)
Bhor, S.A., Sekine, K.-T., Yaeno, T., Yamaoka, N., Nishiguchi, M. and Kobayashi, K
Organizer
平成28年度日本植物病理学会大会
Place of Presentation
岡山コンベンションセンター（岡山県岡山市）
Year and Date
2016-03-21
Related Report
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[Presentation] Construction of a single copy agroinfection vector for the development of infectious cDNA clone of plant viruses2015
Author(s)
Akhter, M.S., Sekine, K.-T., Yaeno, T., Yamaoka, N., Nishiguchi, M. and Kobayashi, K.
Organizer
平成27年度日本植物病理学会関西部会
Place of Presentation
あわぎんホール（徳島県徳島市）
Year and Date
2015-09-29
Related Report
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