| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The present study aimed to generate plants tolerant to plant virus diseases. To this end, we designed a genetically modified plant system to express timely the gene(s) regulating the reactive oxygen species (ROS) production in chloroplasts, which is known to have roles in plant disease development. We established a cheap method to introduce foreign genes into chloroplast and introduced genes that can regulate ROS production into tobacco chloroplasts. Through the examination of a switching gene that turns on the foreign gene in chloroplasts, we found that both protein and RNA structures are important to enable the switching gene product for its efficient transport into chloroplasts.
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