| Project/Area Number |
15K14665
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
Takeda Atsushi 立命館大学, 生命科学部, 准教授 (60560779)
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| Co-Investigator(Renkei-kenkyūsha) |
SATO MASANAO 北海道大学, 農学研究院, 助教 (20517693)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ウイルス由来siRNA / RNAサイレンシング / AGO1 / Nicotiana benthamiana / miRNA |
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| Outline of Final Research Achievements |
In plants, viral infection induces RNA silencing, which is a basal immune response against viruses. In the anti-viral pathways of plant RNA silencing, abundant viral-derived small-interfering RNAs (vsiRNAs) are generated through functions of Dicer-like enzymes (DCLs). A class of 21 nt vsiRNAs cleaved by DCL4 is incorporated into Argonaute 1 (AGO1). The AGO1-vsiRNA complex has a potential to cleave not only viral RNAs but also endogenous mRNAs dependent on the complementarity between target RNAs and vsiRNAs in AGO1. However, determinants of target specificity of AGO1-vsiRNA complex are unknown and thus we cannot predict the target mRNAs downregulated upon viral infection. In this study, we examined the AGO1 specificity in plants experimentally by using a massive transient luciferase assay. We identified several determinants of the plant AGO1 specificity, which can help predict the target mRNAs of vsiRNAs in viral-infected plants in future.
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