| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
The mite Eotetranychus smithi exhibits a facultative diapause that occurs at the egg stage. Diapause was induced by low temperatures alone (<17.5℃) and averted by high temperatures (>=20℃). Transcriptome analysis (RNA-Seq) was conducted to specify the diapause-inducing genes of E. smithi. 85 genes with significantly higher expression were revealed in diapause-egg laying females compared to non-diapause-egg laying females. In order to clarify the relationship between diapause induction and gene expression, we developed an efficient RNAi treatment protocol, resulting in the suppression of genes for 15 days by applying dsRNA solution to a host plant leaf. Although gene expression could be successfully controlled using RNAi, diapause induction was not completely blocked in all of the mites with the ‘particularly high expression' genes suppressed; all females still laid diapause eggs. Other high expression genes may be involved in dormancy in a complex manner.
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