Molecular basis of rice defense against the bacterial leaf blight disease via control of glutamate synthesis in the leaf vasculature

• Project/Area Number: 15K14674
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant nutrition/Soil science
• Research Institution: Tohoku University
• Principal Investigator: HAYAKAWA Toshihiko 東北大学, 農学研究科, 准教授 (60261492)
• Co-Investigator(Renkei-kenkyūsha): TAKAHASHI Hideki 東北大学, 大学院農学研究科, 教授 (20197164) ; KOYAMA Hiroyuki 岐阜大学, 応用生物科学部, 教授 (90234921)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 遺伝子 / 植物 / 生理学 / 病理学 / 発現制御

Outline of Final Research Achievements

Nitrogen nutrition is an essential macronutrient required in quantity for plant growth and productivity. The regulation of glutamate biosynthesis via glutamine-responsive gene expression is important for nitrogen use in rice. It has also been suggested that glutamate biosynthesis in rice may be linked to the pathogenesis of the bacterial leaf blight disease.
Results of this study suggested that the ACT-domain repeat protein 9 (OsACR9), which is composed of domains having homology with the glutamine-binding ACT domains of bacterial glutamine sensors, is associated with glutamine-responsive gene expression of the enzyme related to glutamate biosynthesis and important for nitrogen and carbon metabolisms in rice.

Research Products

• [Journal Article] A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains (2017)
  • Author(s): Yui Yabuki, Miwa Ohashi, Fumi Imagawa, Keiki Ishiyama, Marcel Pascal Beier, Noriyuki Konishi, Toshiko Umetsu-Ohashi, Toshihiko Hayakawa, Tomoyuki Yamaya, Soichi Kojima
  • Journal Title: RICE Volume: 10 Issue: 1 Pages: 1-10
  • DOI: 10.1186/s12284-017-0143-8
  • Peer Reviewed / Open Access
• [Journal Article] Lack of cytosolic glutamine synthetase1;2 activity reduces nitrogen-dependent biosynthesis of cytokinin required for axillary bud outgrowth in rice seedlings (2017)
  • Author(s): Miwa Ohashi, Keiki Ishiyama, Soichi Kojima, Mikiko Kojima, Hitoshi Sakakibara, Tomoyuki Yamaya, Toshihiko Hayakawa
  • Journal Title: Plant and Cell Physiology Volume: 印刷中 Issue: 4 Pages: 679-690
  • DOI: 10.1093/pcp/pcx022
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] コメの生産性を規定するアンモニウム同化系酵素の新規な役割. イネにおける窒素代謝に依存した分げつの成長制御 (2016)
  • Author(s): 大橋美和、小島創一、早川俊彦
  • Journal Title: 化学と生物 Volume: 54 Pages: 867-868
  • Peer Reviewed / Open Access
• [Journal Article] Asparagine synthetase1, but not asparagine synthetase2, is responsible for the biosynthesis of asparagine following the supply of ammonium to rice roots (2015)
  • Author(s): Ohashi M, Ishiyama K, Kojima S, Nakano K, Kanno K, Hayakawa T, Yamaya, T
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 4 Pages: 769-778
  • DOI: 10.1093/pcp/pcv005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 「Mineral transport and sensing in plants」, 4．高濃度アンモニウム供給下の植物の根におけるアンモニウム吸収の負の制御. (2015)
  • Author(s): 高野順平、Kuo-Chen Yeh、河内美樹、早川俊彦、小山博之、時澤睦朋、小林佑理子、神谷岳洋、三輪京子
  • Journal Title: 日本土壌肥料学雑誌 Volume: 86 Pages: 152-158
  • Peer Reviewed
• [Presentation] The protein kinase ACTPK1 down-modulates the high-affinity ammonium uptake of rice roots under high ammonium supply (2017)
  • Author(s): Marcel Pascal Beier, Tsuyoshi Yamanaka, Narumi Tomita, Masataka Ezaki, Toshihiko Hayakawa
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学 (鹿児島県鹿児島市)
• [Presentation] サイトゾル型グルタミン合成酵素1;2を欠損したイネ変異体の分げつの減少はアスパラギンよりもグルタミンの利用可能量の減少に起因する (2017)
  • Author(s): 大橋美和、石山敬貴、小島創一、小西範幸、宮尾光恵、山谷知行、早川俊彦
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学 (鹿児島県鹿児島市)
• [Presentation] シロイヌナズナの根におけるアンモニウムの初期同化 (2016)
  • Author(s): 小西範幸、石山敬貴、菅野圭一、早川俊彦、山谷知行、小島創一
  • Organizer: 第45回根研究集会
  • Place of Presentation: 岡山大学 (岡山県倉敷市)
  • Year and Date: 2016-09-30
• [Presentation] 硝酸供給に応答したシロイヌナズナ根の共生細菌群集の変化 (2016)
  • Author(s): 小西範幸、大久保卓、早川俊彦、山谷知行、南澤究
  • Organizer: 第26回植物微生物研究交流会
  • Place of Presentation: 東北大学 (宮城県仙台市)
  • Year and Date: 2016-09-07
• [Presentation] OsACTPK1 down-modulates the high-affinity NH4+ uptake of rice roots under high NH4+ supply (2016)
  • Author(s): Marcel Pascal Beier, Jin Ishizawa, Haruki Yoshida, Soichi Kojima, Toshihiko Hayakawa
  • Organizer: 第2回植物の栄養研究会
  • Place of Presentation: 名古屋大学 (愛知県名古屋市)
  • Year and Date: 2016-09-02
• [Presentation] Lack of cytosolic glutamine synthease1;2 reduced the availability of glutamine and sucrose for axillary bud outgrowth in the rice seedling. (2016)
  • Author(s): 大橋美和、石山敬貴、草野都、福島敦、小島創一、山谷知行、早川俊彦
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学 (岩手県盛岡市)
  • Year and Date: 2016-03-18
• [Presentation] A serine/threonine/tyrosine protein kinase, OsACTPK1, mediates down-modulation of the high-affinity ammonium uptake by rice roots under high ammonium supply. (2016)
  • Author(s): Marcel P. Beier, Jin Ishizawa, Haruki Yoshida, Soichi Kojima, Toshihiko Hayakawa
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学 (岩手県盛岡市)
  • Year and Date: 2016-03-18
• [Presentation] Severe reduction in the axillary bud outgrowth via metabolic disorder in seedlings of the rice mutant lacking cytosolic glutamine synthetase1;2 (2016)
  • Author(s): Miwa Ohashi, Keiki Ishiyama, Miyako Kusano, Atsushi Fukushima, Soichi Kojima, Atsushi Hanada, Toshihiko Hayakawa, Yoshiya Seto, Junko Kyozuka, Shinjiro Yamaguchi, Tomoyuki Yamaya
  • Organizer: The Third International Symposium on the Nitrogen Nutrition of Plants (Nitrogen 2016)
  • Place of Presentation: the Corum conference center (Montpellier, France)
  • Int'l Joint Research
• [Remarks] 東北大学大学院農学研究科応用生命科学専攻植物機能科学講座植物細胞生化学分野ホームページ
  • URL: http://www.agri.tohoku.ac.jp/cellbio/index-j.htm