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Contribution of "atypical denitrifiers" to denitrification in rice paddy soil

Research Project

Project/Area Number 15K14675
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant nutrition/Soil science
Research InstitutionThe University of Tokyo

Principal Investigator

Senoo Keishi  東京大学, 大学院農学生命科学研究科(農学部), 教授 (40206652)

Co-Investigator(Kenkyū-buntansha) 磯部 一夫  東京大学, 大学院農学生命科学研究科(農学部), 助教 (30621833)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords水田土壌 / 脱窒 / 典型的脱窒細菌 / 非典型的脱窒細菌 / メタトランスクリプトーム解析 / 脱窒反応 / 土壌微生物 / デルタプロテオバクテリア / 鉄還元菌 / 土壌学
Outline of Final Research Achievements

We elucidated the drivers of denitrification in rice paddy soil by metatranscriptomic analysis. It was suggested that typical denitrifiers (denitrifying bacteria belonging to Alpha-, Beta-, or Gammaproteobacteria) largely contributed to nitrite reduction to nitric oxide, whereas atypical denitrifiers largely contributed to other denitrification reactions (i.e., nitrate reduction, nitric oxide reduction and nitrous oxide reduction). Denitrification in rice paddy soil could be “orchestrated” reactions by combination of typical and atypical denitrifiers.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results)

  • [Journal Article] Predominant but Previously-overlooked Prokaryotic Drivers of Reductive Nitrogen Transformation in Paddy Soils, Revealed by Metatranscriptomics2017

    • Author(s)
      Masuda Y, Itoh H, Shiratori Y, Isobe K, Otsuka S, Senoo K.
    • Journal Title

      Microbes and Environments

      Volume: 32 Issue: 2 Pages: 180-183

    • DOI

      10.1264/jsme2.ME16179

    • NAID

      130006789873

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 水田土壌の窒素変換微生物:特定・分離とN2O削減への利用2017

    • Author(s)
      妹尾啓史
    • Organizer
      第34回 土・水研究会
    • Place of Presentation
      つくば農林ホール(茨城県つくば市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 水田土壌オミクス解析から見えた陸域生態系における鉄還元菌の新たな機能-還元的窒素変換(Predominant but previously-unseen prokaryotic drivers of reductive nitrogen transformation in paddy soils, unveiled by metatranscriptomics)2017

    • Author(s)
      増田曜子、伊藤英臣、白鳥豊、磯部一夫、大塚重人、妹尾啓史
    • Organizer
      JpGU-AGU Joint Meeting 2017
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Overlooked but pivotal prokaryotic drivers involved in reductive nitrogen transformations in paddy soils, captured by metatranscriptomics2016

    • Author(s)
      Masuda Y., Itoh H. , Shiratori Y. , Isobe K., Otsuka S., Senoo K.
    • Organizer
      3rd Thunen Symposium on Soil Metagenomics
    • Place of Presentation
      Braunschweig, Germany
    • Year and Date
      2016-12-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 水田土壌に優占する微生物の新機能:鉄還元菌こそが窒素循環に重要である2016

    • Author(s)
      増田曜子、伊藤英臣、白鳥豊、磯部一夫、大塚重人、妹尾啓史
    • Organizer
      日本微生物生態学会第31回大会
    • Place of Presentation
      横須賀市文化会館(神奈川県横須賀市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] 水田土壌に優占するDeltaproteobacteriaの窒素固定・脱窒・DNRAへの寄与-オミクス解析による水田窒素循環微生物コミュニティの刷新-2016

    • Author(s)
      田伏曜子・伊藤英臣・白鳥豊・磯部一夫・大塚重人・妹尾啓史
    • Organizer
      日本土壌微生物学会2016年度大会
    • Place of Presentation
      岐阜大学(岐阜県岐阜市)
    • Year and Date
      2016-06-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] Denitrifying Microbial Community in Agricultural Soils: Key Players Involved in N2O Generation and Elimination2016

    • Author(s)
      Keishi Senoo
    • Organizer
      105th International Symposium and Annual Meeting of the Korean Society for Applied Biological Chemistry
    • Place of Presentation
      Jeju Island, Korea
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Greenhouse gas and soil microorganisms: N2O generating and eliminating microbial communities in agricultural soils2016

    • Author(s)
      KeishiSenoo
    • Organizer
      China-Japan Environmental Microbiology Forum
    • Place of Presentation
      Chongquing, China
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2015-04-16   Modified: 2018-03-22  

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