| Project/Area Number |
15K14680
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 作物学 / 育種学 / ヤムイモ / 施肥技術 / 窒素固定細菌 / 窒素固定菌 |
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| Outline of Final Research Achievements |
Water yam and lesser yam is able to grow under poor fertile soil conditions unless nitrogen fertilization. However, the source of nutrition in survived both yams was not identified in previous study. From the experiment of isolation and estimation of nitrogen-fixing endophytes bacterium using the acetylene reduction activity test and phylogenetic analysis of 16S rRNA gene sequence, 11 species of nitrogen-fixing endophytes bacteria were found in water yam and lesser yam plants. They have related species of the genera Azospirillum, Bacillus, Cohnella, Devosia, Enterobacto, Klebsiella, Pantoea, Ralstonia, Rhizobium, Shinella and Xanthomonas. And more, individual plants of 19 water yam accessions grown in nutrient-poor subsoil, 19 bacterial genera were isolated from plants. This is first report on nitrogen-fixing endophytes bacteria associated with yams.
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