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Study on the symbiosis with ntrogen-fixing endophytes bacterium in yams (Dioscorea spp.)

Research Project

Project/Area Number 15K14680
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant nutrition/Soil science
Research InstitutionTokyo University of Agriculture

Principal Investigator

Hironobu Shiwachi  東京農業大学, 国際食料情報学部, 教授 (40385505)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords作物学 / 育種学 / ヤムイモ / 施肥技術 / 窒素固定細菌 / 窒素固定菌
Outline of Final Research Achievements

Water yam and lesser yam is able to grow under poor fertile soil conditions unless nitrogen fertilization. However, the source of nutrition in survived both yams was not identified in previous study. From the experiment of isolation and estimation of nitrogen-fixing endophytes bacterium using the acetylene reduction activity test and phylogenetic analysis of 16S rRNA gene sequence, 11 species of nitrogen-fixing endophytes bacteria were found in water yam and lesser yam plants. They have related species of the genera Azospirillum, Bacillus, Cohnella, Devosia, Enterobacto, Klebsiella, Pantoea, Ralstonia, Rhizobium, Shinella and Xanthomonas. And more, individual plants of 19 water yam accessions grown in nutrient-poor subsoil, 19 bacterial genera were isolated from plants. This is first report on nitrogen-fixing endophytes bacteria associated with yams.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (18 results)

All 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 7 results) Presentation (9 results) (of which Invited: 2 results) Remarks (1 results)

  • [Int'l Joint Research] 国際熱帯農業研究所(ナイジェリア)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Intra Specific Genetic Diversity in Dioscorea alata accessions collected from Myanmar, Papua New Guinea and Japan.2018

    • Author(s)
      Favour Olu-Olusegun, Pachakkil Babil, Hidehiko Kikuno, Kenji Irie, Hidekazu Toyohara and Hironobu Shiwachi
    • Journal Title

      The International Society for Southeast Asian Agricultural Sciences

      Volume: 23

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of the source of nitrogen during water yam (Dioscorea alata L.) growth using δ15N observations2018

    • Author(s)
      Kanako Takada, Hidehiko Kikuno, Pachakkil Babil and Hironobu Shiwachi
    • Journal Title

      Tropical Agriculture and Development

      Volume: 62

    • NAID

      130007497597

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitrogen-fixing Endophytic Bacteria is involved with the lesser yam (Dioscorea esculenta L.) growth under low fertile soil condition2017

    • Author(s)
      Abdul Qayom Rezaei, Hidehiko Kikuno, Pachakkil Babil, Naoto Tanaka, Byoung-Jae Parak, Michio Onjo and Hironobu Shiwachi
    • Journal Title

      Tropical Agriculture and Development

      Volume: 61 Pages: 40-47

    • NAID

      130006900277

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Water yam (Dioscorea alata L.) is able to grow under low fertile soil2017

    • Author(s)
      5.Knako Takada, Hidehiko Kikuno, Pachakkil Babil, Kenji Irie and Hironobu Shiwachi
    • Journal Title

      Tropical Agriculture and Development

      Volume: 61 Pages: 8-14

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Water yam (Dioscorea alata L.) is able to grow in low fertile soil conditions.2017

    • Author(s)
      Kanako Takada, Hidehiko Kikuno, Pachakkil Babil, Kenji Irie and Hironobu Shiwachi.
    • Journal Title

      Tropical Agriculture and Development

      Volume: 62

    • NAID

      130006900275

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of Nitrogen Fertilizer on Growth of the Lesser Yam (Dioscorea esculenta L.)2016

    • Author(s)
      Abdul Qayom Rezaei, Hidehiko Kikuno, Kaoru Sugiyama, Pachakkil Babil, Byoung-Jae Parak, Michio Onjo and Hironobu Shiwachi.
    • Journal Title

      Tropical Agriculture and Development

      Volume: 60 Pages: 185-190

    • NAID

      130005464651

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of Nitrogen Fertilizer on Growth of the Lesser Yam (Dioscorea esculenta L.)2016

    • Author(s)
      Abdul Qayom Rezaei, Hidehiko Kikuno, Kaoru Sugiyama, Pachakkil Babil, Byoung-Jae Park, Michio Onjo and Hironobu Shiwachi
    • Journal Title

      Tropical Agriculture and Development

      Volume: 61

    • NAID

      130005464651

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 熱帯産ヤムイモ(Dioscorea spp.)の生産性の改善に関する研究2017

    • Author(s)
      志和地弘信
    • Organizer
      日本熱帯農業学会第121回講演会
    • Place of Presentation
      日本大学生物資源科学部(神奈川県藤沢市)
    • Year and Date
      2017-03-11
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] SSR marker based genetic diversity in water yam (Dioscorea alata)2017

    • Author(s)
      Favour Olu-Olusegun, Pachakkil Babil, Hidehiko Kikuno and Hironobu Shiwachi
    • Organizer
      日本熱帯農業学会第122回講演会 香川大学
    • Related Report
      2017 Annual Research Report
  • [Presentation] Agronomic Evaluation of Improved Water Yam (Dioscorea alata) Varieties with Tuber Characteristics2017

    • Author(s)
      Theresa Ige, Hidehiko Kikuno, Pachakki Babil, Antonio Lopez-Montes and Hironobu Shiwachi
    • Organizer
      日本熱帯農業学会第122回講演会 香川大学
    • Related Report
      2017 Annual Research Report
  • [Presentation] ダイジョ(D. alata L.)における窒素固定細菌の共生機序に関する研究2017

    • Author(s)
      三輪菜都美・菊野日出彦・田中尚人・志和地弘信
    • Organizer
      日本熱帯農業学会第122回講演会 香川大学
    • Related Report
      2017 Annual Research Report
  • [Presentation] Diversity of endophytic diazotrophs associated with yam (Dioscorea spp.)2017

    • Author(s)
      Ouyabe, M., Babil, P., Kikuno, H., Tanaka, N. and Shiwachi, H
    • Organizer
      日本熱帯農業学会第122回講演会 香川大学
    • Related Report
      2017 Annual Research Report
  • [Presentation] アフリカにおける緑の革命には孤児作物(Orphan Crop)研究がカギになる。2016

    • Author(s)
      志和地弘信
    • Organizer
      日本熱帯農業学会第120回講演会
    • Place of Presentation
      鹿児島大学農学部(鹿児島県鹿児島市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Nitrogen-fixing Endophytic Bacteria associated with the lesser yam (Dioscorea esculenta L.)2016

    • Author(s)
      Abdul Qayom Rezaei, Hidehiko Kikuno, Pachakkil Babil, Naoto Tanaka, Byoung-Jae Parak, Michio Onjo and Hironobu Shiwachi.
    • Organizer
      日本熱帯農業学会第120回講演会
    • Place of Presentation
      鹿児島大学農学部(鹿児島県鹿児島市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Research-status Report
  • [Presentation] Effect of Nitrogen Fertilizer on Growth of the Lesser Yam (Dioscorea esculenta L.)2016

    • Author(s)
      Abdul Qayom Rezaei, Hidehiko Kikuno, Kaoru Sugiyama, Pachakkil Babil, Byoung-Jae Park, Michio Onjo and Hironobu Shiwachi
    • Organizer
      日本熱帯農業学会第119回講演会
    • Place of Presentation
      明治大学農学部
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report
  • [Presentation] ダイジョに内生する窒素固定細菌に関する研究2016

    • Author(s)
      髙田花奈子, 菊野日出彦, 池永誠, 境雅夫, 遠城道雄, 朴炳宰, Babil Pachakkil, 志和地弘信
    • Organizer
      日本熱帯農業学会第119回講演会
    • Place of Presentation
      明治大学農学部
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report
  • [Remarks] 情熱広場

    • URL

      http://www.nodai.ac.jp/int/develop/index.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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