| Project/Area Number |
15K14681
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
Kuchitsu Kazuyuki 東京理科大学, 理工学部応用生物科学科, 教授 (50211884)
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| Co-Investigator(Kenkyū-buntansha) |
来須 孝光 東京工科大学, 応用生物学部, 助教 (50422499)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | オートファジー / イネ / 脂質 / 登熟 / リサイクル / 病虫害抵抗性 / 胚乳 / タペート細胞 / 種子登熟 / 澱粉 / 花粉 / 雄性不稔 |
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| Outline of Final Research Achievements |
Autophagy, one of the major catabolic pathways in eukaryotic cells, plays critical roles in recycling of proteins and metabolites including lipids, and is involved in many physiological processes including abiotic and biotic stress responses, maintenance of cellular homeostasis, efficient nutrient remobilization at the whole-plant level and biomass production in plants. However, its roles in reproductive development had remained poorly understood. Rice autophagy-deficient mutants showed sporophytic male sterility, failed to accumulate lipidic and starch components in pollen grains at the flowering stage, showed reduced pollen germination activity, and had limited anther dehiscence. We showed that sugar and starch metabolism during seed maturation is also affected in the autophagy-deficient mutant of rice. Phenotypic, microscopic and proteomic analyses revealed critical roles of autophagy in seed development and quality of grains in rice.
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