Electrochemical screening of redox enzymes

• Project/Area Number: 15K14684
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: University of Tsukuba
• Principal Investigator: TSUJIMURA Seiya 筑波大学, 数理物質系, 准教授 (30362429)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 酸化還元酵素 / 酵素電極 / 多孔質炭素 / スクリーニング / 電極触媒 / ハイドロゲル / メソ孔 / マクロ孔 / 印刷電極 / メディエータ / 酵素電池 / ホフマイスター / ポリマー

Outline of Final Research Achievements

Hierarchically structured carbons composed of mesoporous and macroporous were developed for enzyme electrode: the large surface area of mesoporous materials can increase the enzyme loading and electron transfer efficiency, and the macropores enable the efficient mass transport. The porous carbon electrode allows the development of highly efficient screening system of redox enzymes for the application of electrocatalyst.

Research Products

• [Int'l Joint Research] CRPP, CNRS，Bordeaux/IBP，CNRS, Marseille/Univ. Grenoble-alps(フランス)
• [Int'l Joint Research] CRPP(フランス)
• [Journal Article] Bimolecular Rate Constants for FAD-dependent Glucose Dehydrogenase from Aspergillus terreus and Organic Electron Acceptors (2017)
  • Author(s): Tsuruoka, N., Sadakane, T., Hayashi, R., Tsujimura, S.
  • Journal Title: Int. J. Mol. Sci. Volume: 18 Issue: 3 Pages: 604-604
  • DOI: 10.3390/ijms18030604
  • NAID: 120007134939
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Lowering the potential of electroenzymatic glucose oxidation on redox hydrogel-modified porous carbon electrode (2017)
  • Author(s): Suzuki, A., Mano, N., Tsujimura, S.
  • Journal Title: Electrochim. Acta Volume: 232 Pages: 581-585
  • DOI: 10.1016/j.electacta.2017.03.007
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Screen-printed, Paper-based, Array-type, Origami Biofuel Cell (2017)
  • Author(s): Shitanda, I., Kato, S., Tsujimura, S., Hoshi, Y., Itagaki, M.
  • Journal Title: Chemistry Letters Volume: 46 Issue: 5 Pages: 726-728
  • DOI: 10.1246/cl.170047
  • NAID: 130005655632
  • ISSN: 0366-7022, 1348-0715
  • Peer Reviewed / Open Access
• [Journal Article] Hierarchical meso/macro-porous carbon fabricated from dual MgO templates for direct electron transfer enzymatic electrodes (2017)
  • Author(s): Funabashi, H., Takeuchi, S., Tsujimura, S.
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 45147-45147
  • DOI: 10.1038/srep45147
  • NAID: 120007134892
  • Peer Reviewed / Open Access
• [Journal Article] Redox-Polymers Enable Uninterrupted Day/Night Photo-Driven Electricity Generation in Biophotovoltaic Devices (2017)
  • Author(s): Darus, L., Sadakane, T., Ledezma, P., Tsujimura, S., Osadebe, I., Leech, D., Gorton, L., Freguia, S.
  • Journal Title: J. Electrochem. Soc. Volume: 164 Issue: 3 Pages: H3037-H3040
  • DOI: 10.1149/2.0091703jes
  • NAID: 120007129199
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hofmeister effects on the glucose oxidase hydrogel-modified electrode (2016)
  • Author(s): Suzuki, A., Tsujimura, S.
  • Journal Title: Electrochim. Acta Volume: 201 Pages: 228-232
  • DOI: 10.1016/j.electacta.2016.03.116
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Long-term Continuous Operation of FAD-dependent Glucose Dehydrogenase Hydrogel-modified Electrode at 37 °C (2016)
  • Author(s): Suzuki, A., Tsujimura, S.
  • Journal Title: Chemistry Letters Volume: 45 Issue: 4 Pages: 484-486
  • DOI: 10.1246/cl.160053
  • NAID: 130005143997
  • ISSN: 0366-7022, 1348-0715
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Templated mesoporous carbons: Synthesis and applications (2016)
  • Author(s): Inagaki, M., Toyoda, M., Soneda, Y., Tsujimura, S., Morishita, T.
  • Journal Title: Carbon Volume: 107 Pages: 448-448
  • DOI: 10.1016/j.carbon.2016.06.003
  • Peer Reviewed
• [Journal Article] Redox Hydrogel of Glucose Oxidase on MgO-Templated Carbon Electrode (2016)
  • Author(s): Suzuki, A., Murata, K., Mano, N., Tsujimura, S.
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 89 Issue: 1 Pages: 24-26
  • DOI: 10.1246/bcsj.20150252
  • NAID: 130005119452
  • ISSN: 0009-2673, 1348-0634
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Electrochemical Oxygen Reduction Catalyzed by Bilirubin Oxidase with the Aid of 2,2'-Azinobis(3-ethylbenzothiazolin-6-sulfonate) on a MgO-template Carbon Electrode (2015)
  • Author(s): Tsujimura, S., Murata, K.
  • Journal Title: Electrochim. Acta Volume: 180 Pages: 555-559
  • DOI: 10.1016/j.electacta.2015.08.156
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Electrochemical Activation of a Novel Laccase, MELAC, Isolated from Compost (2015)
  • Author(s): Murata, K., Shigemori, Y., Tsujimura, S.,
  • Journal Title: Chem. Lett. Volume: 44 Issue: 5 Pages: 654-655
  • DOI: 10.1246/cl.150057
  • NAID: 130005067967
  • Peer Reviewed / Open Access
• [Journal Article] Effect of Pore Size of MgO-templated Carbon on the Direct Electrochemistry of <small>D</small>-fructose Dehydrogenase (2015)
  • Author(s): Funabashi, H., Murata, K., Tsujimura, S.,
  • Journal Title: Electrochemistry Volume: 83 Issue: 5 Pages: 372-375
  • DOI: 10.5796/electrochemistry.83.372
  • NAID: 130005067089
  • ISSN: 1344-3542, 2186-2451
  • Peer Reviewed / Open Access
• [Journal Article] Electrochemical Impedance Simulation of Branch Structure Porous Carbon Electrode Using Transmission Line Model (2015)
  • Author(s): Shitanda, I., Tsujimura, S., Yanai, H., Hoshi, Y., Itagaki, M.,
  • Journal Title: Electrochemistry Volume: 83 Issue: 5 Pages: 335-338
  • DOI: 10.5796/electrochemistry.83.335
  • NAID: 130005067105
  • ISSN: 1344-3542, 2186-2451
  • Peer Reviewed / Open Access
• [Journal Article] Electrochemical Impedance Study of Screen-printed Branch Structure Porous Carbon Electrode using MgO-templated Carbon and MgO Particle and its Application for Bilirubin Oxidase-immobilized Biocathode (2015)
  • Author(s): Shitanda, I., Nakafuji, H., Tsujimura, S., Hoshi, Y., Itagaki, M.,
  • Journal Title: Electrochemistry Volume: 83 Issue: 5 Pages: 329-331
  • DOI: 10.5796/electrochemistry.83.329
  • NAID: 130005067106
  • ISSN: 1344-3542, 2186-2451
  • Peer Reviewed / Open Access
• [Presentation] マクロ-メソ階層構造を有する酸化マグネシウム鋳型炭素の酵素電極への応用 (2017)
  • Author(s): 船橋 広人,竹内 聖詞, 辻村 清也
  • Organizer: 第84回電気化学会大会
  • Place of Presentation: 首都大東京、東京都八王子市
  • Year and Date: 2017-03-25
• [Presentation] 酸化マグネシウム鋳型炭素を用いたビリルビンオキシダーゼ酸素還元カソード (2017)
  • Author(s): 石居 周二,船橋 広人,辻村 清也
  • Organizer: 第84回電気化学会大会
  • Place of Presentation: 首都大東京、東京都八王子市
  • Year and Date: 2017-03-25
• [Presentation] 酵素型バイオ燃料電池の最新動向 (2017)
  • Author(s): 辻村清也
  • Organizer: 電気化学セミナー 最先端電池技術2017
  • Place of Presentation: タワーホール船堀、東京都江戸川区
  • Year and Date: 2017-01-20
  • Invited
• [Presentation] FAD 依存性グルコース脱水 素酵素と電子受容体との反 応 に及ぼす電解質の影響 (2016)
  • Author(s): 安立 翼， 椙原和法，辻村清也
  • Organizer: 第 6 2 回ポーラログラフィーおよび電気分析化学討論会
  • Place of Presentation: 宮古島市中央公民館大ホール、沖縄県宮古島市
  • Year and Date: 2016-11-19
• [Presentation] Enzymatic biofuel cell based on porous carbon materials (2016)
  • Author(s): Seiya Tsujimura
  • Organizer: PRiME2016
  • Place of Presentation: Hawaii Convention Center、Honolulu, HI, USA
  • Year and Date: 2016-10-03
  • Int'l Joint Research / Invited
• [Presentation] Designing Macro-Meso Pore Structure of MgO-Templated Carbon for Det-Based Enzyme Electrodes (2016)
  • Author(s): Hiroto Funabashi, Seiya Tsujimura
  • Organizer: PRiME2016
  • Place of Presentation: Hawaii Convention Center、Honolulu, HI, USA
  • Year and Date: 2016-10-03
  • Int'l Joint Research
• [Presentation] Glucose/O2 Biofuel Cell Constructed from MgO-Temlated Carbon Modified Carbon Cloth (2016)
  • Author(s): Ayumu Niiyama, Seiya Tsujimura
  • Organizer: PRiME2016
  • Place of Presentation: Hawaii Convention Center、Honolulu, HI, USA
  • Year and Date: 2016-10-03
  • Int'l Joint Research
• [Presentation] 水系（生体関連）有機レドックス反応とバイオ燃料電池への応用 (2016)
  • Author(s): 辻村清也
  • Organizer: 新レドックス系エネルギー技術研究ＷＧ 第一回講演会
  • Place of Presentation: アルス市ヶ谷、東京都千代田区
  • Year and Date: 2016-09-13
  • Invited
• [Presentation] Enzymatic biofuel cell based on porous carbon materials (2016)
  • Author(s): Seiya Tsujimura
  • Organizer: France-Japan Meeting on (bio)-functionalization of nanostructured carbon and (bio)-electrocatalytic applications
  • Place of Presentation: Univ. Grenoble-Alpes, Grenoble、France
  • Year and Date: 2016-09-01
  • Int'l Joint Research / Invited
• [Presentation] Enzymatic biofuel cell based on MgO-templated carbon (2016)
  • Author(s): Seiya Tsujimura
  • Organizer: Seminar，Bioenergetique et Ingenierie des Proteines
  • Place of Presentation: BIP、Marseilles、France
  • Year and Date: 2016-08-29
  • Int'l Joint Research / Invited
• [Presentation] Glucose/O2 enzymatic biofuel cell based on MgO-templated porous carbon (2016)
  • Author(s): Seiya Tsujimura, Ayumu Niiyama
  • Organizer: ISE
  • Place of Presentation: World Forum, The Hague,The Netherlands
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] 酵素電池の開発 (2016)
  • Author(s): 辻村清也
  • Organizer: TIMS研究交流会
  • Place of Presentation: 筑波大、茨城県つくば市
  • Year and Date: 2016-07-01
  • Invited
• [Presentation] Enzyme-Based Biofuel Cell (2016)
  • Author(s): Seiya Tsujimura
  • Organizer: The Japan-America Frontiers of Engineering 2016
  • Place of Presentation: ベックマンセンター、アーバイン、アメリカ
  • Year and Date: 2016-06-15
  • Int'l Joint Research / Invited
• [Presentation] MgO - templated mesoporous carbon for enzyme electrode (2016)
  • Author(s): Seiya Tsujimura
  • Organizer: Elecnano7
  • Place of Presentation: Lille University、Lille、France
  • Year and Date: 2016-05-23
  • Int'l Joint Research
• [Presentation] 酵素燃料電池の開発 (2016)
  • Author(s): 辻村清也
  • Organizer: 第118回 有機エレクトロニクス研究センター講演会
  • Place of Presentation: 山形大有機エレクトロニクス研究センター、山形県米沢市
  • Year and Date: 2016-04-26
  • Invited
• [Presentation] Hofmeister Effects on Glucose oxidase-Redox Hydrogel Electrode (2016)
  • Author(s): Seiya Tsujimura, Aimi Suzuki
  • Organizer: 19th Topical Meeting of the International Society of Electrochemistry
  • Place of Presentation: The University of Auckland、オークランド、ニュージーランド
  • Year and Date: 2016-04-17
  • Int'l Joint Research
• [Presentation] メソ・マクロ階層構造を有する多孔質炭素を用いた酵素機能電極の特性評価 (2016)
  • Author(s): 船橋 広人,辻村 清也
  • Organizer: 電気化学会第83回大会
  • Place of Presentation: 大阪大学（大阪市，吹田市）
  • Year and Date: 2016-03-29
• [Presentation] レドックスハイドロゲル型グルコース酸化電極に対する電解質の影響 (2016)
  • Author(s): 辻村 清也,鈴木 愛未
  • Organizer: 電気化学会第83回大会
  • Place of Presentation: 大阪大学（大阪市，吹田市）
  • Year and Date: 2016-03-29
• [Presentation] おむつに搭載する新規自己駆動型尿糖バイオセンサ作製と出力評価 (2016)
  • Author(s): 野原 早貴,星 芳直,四反田 功,辻村 清也,板垣 昌幸
  • Organizer: 電気化学会第83回大会
  • Place of Presentation: 大阪大学（大阪市，吹田市）
  • Year and Date: 2016-03-29
• [Presentation] MgO鋳型炭素修飾カーボンクロス電極を用いた酵素燃料電池の特性評価 (2016)
  • Author(s): 新山 歩,大場 貴子,辻村 清也
  • Organizer: 電気化学会第83回大会
  • Place of Presentation: 大阪大学（大阪市，吹田市）
  • Year and Date: 2016-03-29
• [Presentation] 糖および乳酸を燃料としたバイオ燃料電池アレイの作製と評価 (2016)
  • Author(s): 岩下 梨沙,籾山 美咲,星 芳直,四反田 功,板垣 昌幸,辻村 清也
  • Organizer: 電気化学会第83回大会
  • Place of Presentation: 大阪大学（大阪市，吹田市）
  • Year and Date: 2016-03-29
• [Presentation] Enzymatic biofuel cell based on porous carbon materials (2015)
  • Author(s): Tsujimura, S.
  • Organizer: MANA-RSC symposium Materials for Energy Generation and Storage
  • Place of Presentation: NIMS（MANA棟）（茨城県，つくば市）
  • Year and Date: 2015-10-15
  • Int'l Joint Research / Invited
• [Presentation] ウェアラブルデバイスの電源に利用可能な薄膜型バイオ燃料電池アレイの開発 (2015)
  • Author(s): 籾山美咲・星 芳直・辻村清也・四反田功・板垣昌幸
  • Organizer: 第5回CSJ化学フェスタ
  • Place of Presentation: タワーホール船堀（東京都，江戸川区）
  • Year and Date: 2015-10-13
• [Presentation] 多孔質炭素－カーボンクロス複合電極を用いた酵素燃料電池の開発 (2015)
  • Author(s): 新山 歩・村田一樹・辻村清也
  • Organizer: 第5回CSJ化学フェスタ
  • Place of Presentation: タワーホール船堀（東京都，江戸川区）
  • Year and Date: 2015-10-13
• [Presentation] 多孔質炭素修飾酵素電極における細孔構造の検討 (2015)
  • Author(s): 船橋広人・辻村清也
  • Organizer: 第5回CSJ化学フェスタ
  • Place of Presentation: タワーホール船堀（東京都，江戸川区）
  • Year and Date: 2015-10-13
• [Presentation] Biocathode based on a MgO-template Carbon Electrode Modified with ABTS and Bilirubin Oxidase (2015)
  • Author(s): Tsujimura, S.
  • Organizer: the 66th of Annual meeting of International Society of Electrochemistry
  • Place of Presentation: Taipei，台湾
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] Fabrication of Paper-based Disk-shaped Glucose Biofuel Array (2015)
  • Author(s): Isao Shitanda, Saki Nohara, Seiya Tsujimura, Yoshinao Hoshi, Masayuki Itagaki
  • Organizer: the 66th of Annual meeting of International Society of Electrochemistry
  • Place of Presentation: Taipei，台湾
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] Characterization of Glucose Oxidase-Redox Hydrogel on MgO-templated Carbon Electrode (2015)
  • Author(s): Aimi Suzuki, Kazuki Murata, Seiya Tsujimura
  • Organizer: the 66th of Annual meeting of International Society of Electrochemistry
  • Place of Presentation: Taipei，台湾
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] Glucose Biosensor based on a Glassy Carbon Electrode Modified with Poly(methylene green) and FADdependent Glucose Dehydrogenase (2015)
  • Author(s): Nozomu Tsuruoka, Kazuki Murata, Seiya Tsujimura
  • Organizer: the 66th of Annual meeting of International Society of Electrochemistry
  • Place of Presentation: Taipei，台湾
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] カーボンクロスとMgO鋳型炭素を用いたバイオカソードの電気化学インピーダンス測定による特性評価 (2015)
  • Author(s): 岩下 梨沙,星 芳直,四反田 功,村田 一樹,辻村 清也,板垣 昌幸
  • Organizer: 2015年電気化学秋季大会
  • Place of Presentation: 埼玉工業大学（埼玉県，深谷市）
  • Year and Date: 2015-09-11
• [Presentation] 紙を基板とした円盤状バイオ燃料電池の作製と尿糖電池への応用 (2015)
  • Author(s): 野原 早貴,星 芳直,四反田 功,辻村 清也,板垣 昌幸
  • Organizer: 2015年電気化学秋季大会
  • Place of Presentation: 埼玉工業大学（埼玉県，深谷市）
  • Year and Date: 2015-09-11
• [Presentation] 多孔質炭素-カーボンクロス複合電極を用いた酵素燃料電池の開発 (2015)
  • Author(s): 新山 歩,村田 一樹,辻村 清也
  • Organizer: 2015年電気化学秋季大会
  • Place of Presentation: 埼玉工業大学（埼玉県，深谷市）
  • Year and Date: 2015-09-11
• [Presentation] 電解質のイオン特性を利用したグルコース脱水素酵素の安定化 (2015)
  • Author(s): 椙原 和法,安立 翼,辻村 清也
  • Organizer: 2015年電気化学秋季大会
  • Place of Presentation: 埼玉工業大学（埼玉県，深谷市）
  • Year and Date: 2015-09-11
• [Presentation] グルコース脱水素酵素のメディエータ型電極反応に対する電解質の影響 (2015)
  • Author(s): 安立 翼,椙原 和法,村田 一樹,辻村 清也
  • Organizer: 2015年電気化学秋季大会
  • Place of Presentation: 埼玉工業大学（埼玉県，深谷市）
  • Year and Date: 2015-09-11
• [Presentation] ドックスハイドロゲル修飾多孔質グルコース酸化電極の高出力化・安定化 (2015)
  • Author(s): 鈴木 愛未,辻村 清也
  • Organizer: 2015年電気化学秋季大会
  • Place of Presentation: 埼玉工業大学（埼玉県，深谷市）
  • Year and Date: 2015-09-11
• [Presentation] Recent advances in carbon electrodes for the development of enzyme-based biofuel cell (2015)
  • Author(s): Tsujimura, S.
  • Organizer: SMOBE-2015
  • Place of Presentation: Univ. Antwapen，ベルギー
  • Year and Date: 2015-08-15
  • Int'l Joint Research / Invited
• [Remarks] 筑波大学大学院数理物質科学研究科物性・分子工学専攻生物電気化学研究室
  • URL: http://www.ims.tsukuba.ac.jp/~tsujimura_lab/index.html
• [Patent(Industrial Property Rights)] 発電デバイス、発電方法及び濃度測定方法 (2016)
  • Inventor(s): 四反田功，辻村清也
  • Industrial Property Rights Holder: 四反田功，辻村清也
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-195054
  • Filing Date: 2016-09-30