| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
To reveal the mechanisms determining plasmid behavior in nature, we assessed the transferability of plasmids from one donor strain to either of two recipient candidates as the most simplified example of conjugation in consortia. Two different Pseudomonas strains were used as model hosts and pCAR1, NAH7, pB10, and R388 were used as model plasmids. Plasmids were generally transferred more frequently to the same species than to different. The coexistence of other recipients enhanced such tendency to be transferred to the same species more frequently, while pCAR1 transfer was less affected.
To clarify the factor(s) affecting such phenomena, we constructed a screening method with BAC library of recipient chromosomal genome. It was suggested that there were some regions which accelerate plasmid recruitment from the same species. We also constructed the screening method of transposon inserted library of NAH7 in order to search the factor encoded on plasmid, and obtained 8 candidates.
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