| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
On exposure to cold starvation, Escherichia coli falls into a viable but non-culturable state, where colony formation is lost in spite of retaining signs of viability. We hypothesized that, during cold starvation, specific gene expressions important for colony formation are diminished and that boosting such gene functions would restrain cells from falling into the low-colony state. From the ASKA library containing all E. coli ORFs, we screened such genes restoring the diminished functions.
When seven such candidate clones are put in competition in the ability to survive against cold starvation, the winner was cpdA, encoding cAMP phosphodiesterase, suggesting that cAMP is a negative regulator in colony formation. In fact, cyaA or crp mutants kept high colony formation for 30 days in starvation. Assuming that a critical gene determining colony formation is under the control of cAMP-CRP, we are seeking it by RNA-seq, though too many genes change their expressions in response to cAMP-CRP
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