| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Deep-sea environments are characterized by high pressure and poor nutrients, and efficient nitrogen uptake is crucial for survival of organisms under the extreme condition. In this study, we focus on the functionality of peptide transporters in deep-sea bacteria, and elucidate the mechanism of their high-pressure adaptation. The genes encoding peptide transporters were cloned and the transporter proteins were successfully expressed in Escherichia coli. They were purified by affinity chromatography and will be subject to reconstitution in lipid vesicles. The peptide transport activity was analyzed using β-Ala-Lys (AMCA) as a substrate. We found that the activity was inhibited by CCCP, suggesting that the peptide transport is coupled with proton gradient across the plasma membrane.
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