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Specific secretory production system of D-amino acid derivatives by budding yeast and its application for their fermentation production

Research Project

Project/Area Number 15K14699
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionRitsumeikan University

Principal Investigator

WAKAYAMA MAMORU  立命館大学, 生命科学部, 教授 (70240455)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsD-アミノ酸 / 出芽酵母 / N-アセチル-D-アミノ酸 / D-アミノアシラーゼ / アミノ酸ラセマーゼ / D-アミノアセチルトランスフェラーゼ / 発酵生産 / D-アミノ酸アセチルトラン スフェラーゼ / D-アミノ酸アセチルトランスフェラーゼ / N-アセチル-D-アミノ酸
Outline of Final Research Achievements

D-amino acids might be the presence of the shade in comparison with L-amino acids. Recently, however, it has been clarified that some D-amino acids play essential roles in the brain functions in mammals. In addition, therapeutic drugs such as antibiotics require D-amino acids as components. In this research project, potential fermentation production of D-amino acid derivatives has been investigated and its feasibility as one of the D-amino acid production methods has been evaluated. We showed the result that D-amino acid derivatives produced by fermentation method were enzymatically converted to D-amino acids.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2017 2015

All Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Presentation] 機能性食品添加剤への利用に向けた新規D-アミノ酸誘導体生産方法の開発2017

    • Author(s)
      中本 侃、若山 守
    • Organizer
      第71回日本栄養・食糧学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 出芽酵母を利用したD-アミノ酸誘導体生産系の構築2017

    • Author(s)
      中本 侃, 梅川 碧里, 林 順司, 若山 守
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Construction of D-amino acid derivative production system using Saccharomyces cerevisiae2017

    • Author(s)
      Akira Nakamoto, Midori Umekawa, Junji Hayashi, Mamoru Wakayama
    • Organizer
      New Zealand Microbiological Society Conference 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Purification and characterization of D-amino acid oxidase from Aspergillus sp. GI-32015

    • Author(s)
      A. Kariyone, S. Yano, K. Takagi, M. Wakayama
    • Organizer
      2015 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2015-12-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2021-01-27  

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