Operation of nuclear actin filaments and its application to the analysis of gene reprogramming mechanisms
| Project/Area Number |
15K14706
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MITANI Tasuku 近畿大学, 先端技術総合研究所, 教授 (10322265)
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| Research Collaborator |
Heinis Christian École Polytechnique Fédérale de Lausanne
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 細胞核 / クロマチン / 遺伝子発現 / アクチンファミリー / エピジェネティクス / 遺伝子初期化 / アクチン / 核内アクチン / クロマチンリモデリング |
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| Outline of Final Research Achievements |
In this project, we showed that artificial nuclear F-actin associates with chromatin and that nuclear F-actin affects Wnt/beta-catenin signaling, which has important roles on gene reprogramming and cell development. Indeed, the expression of OCT4 is shown to be regulated by nuclear F-actin. By screening a peptide library, we identified bicyclic peptides binding to G- or F-actin. We also showed a possibility that these bicyclic peptides are useful for artificial regulation of nuclear F-actin.
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Report
(4 results)
Research Products
(81 results)
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[Journal Article] Actin Family Proteins in the Human INO80 Chromatin Remodeling Complex Exhibit Functional Roles in the Induction of Heme Oxygenase-1 with Hemin.2017
Author(s)
Takahashi, Y., Murakami, H., Akiyama, Y., Katoh, Y., Oma, Y., Nishijima, H., Shibahara, K.I., Igarashi, K., Harata, M.
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Journal Title
Front. Genet.
Volume: 印刷中
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] The actin family protein ARP6 contributes to the structure and the function of the nucleolus2015
Author(s)
Kitamura, H., Matsumori, H., Kalendova, A., Hozak, P., Goldberg, I.G., Nakao, M., Saitoh, N., Harata, M
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Journal Title
BBRC
Volume: 464
Issue: 2
Pages: 554-560
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Analyses of molecular evolution of the histone variant H2A.Z2017
Author(s)
S. Kitagawa, M. Kusakabe, H. Oku, D. Takahashi, T. Narimiya, Y. Nakabayashi, M. Seki, M. Harata
Organizer
Tohoku Forum for Creativity Thematic Program 2017 New Horizons in Food Science via Agricultural Immunology, Stage 2 International symposium and youth program, Food Safety and Functional Evaluation
Related Report
Int'l Joint Research
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[Presentation] Functional analysis of the contribution of acetylation of H2A.Z to epigenetic regulations2015
Author(s)
Masayuki Kusakabe, Ryo Matsuda, Hiroyuki Oku, Tetsuya Hori, Tatsuo Fukagawa, Hitoshi Kurumizaka, Masahiko Harata
Organizer
International Symposium on Chromatin Structure, Dynamics, and Function
Place of Presentation
Awaji Yumebutai International Conference Center, Awaji, Hyogo, Japan
Year and Date
2015-08-23
Related Report
Int'l Joint Research
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[Presentation] Roles of Actin Family Proteins in the Organization of Functional Structures within the Nucleus2015
Author(s)
Masahiko Harata, Shota Yamazaki, Hiroshi Kitamura, Koji Yamamoto, Yukako Oma Yuki Orihara, Haruka Matsumori, Noriko Saitoh, and Masahiko Harata
Organizer
International Symposium on Chromatin Structure, Dynamics, and Function
Place of Presentation
Awaji Yumebutai International Conference Center, Awaji, Hyogo, Japan
Year and Date
2015-08-23
Related Report
Int'l Joint Research
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