Structural and functional analyses for the transcription factor complex in TGF-beta signalling.

Research Project

Project/Area Number	15K14708
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Applied biochemistry
Research Institution	The University of Tokyo
Principal Investigator	Miyazono Kenichi 東京大学, 大学院農学生命科学研究科(農学部), 特任助教 (90554486)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	TGF-β / 転写因子 / X線結晶構造解析 / 構造解析 / 細胞分化
Outline of Final Research Achievements	The transforming growth factor beta (TGF-beta) superfamily of cytokines regulates a number of biological processes such as cell self-renewal, differentiation, apoptosis and senescence. In this study, we focused on the transcription factor complex (Smad3-FoxH1) that is formed in cells by the stimulation of TGF-beta signal. The Smad3-FoxH1 complex regulates a number of gene expressions to regulate the differentiation of stem cells. Smad3 and FoxH1 from human were overexpressed in E. coli. The structure of Smad3-FoxH1 complex was determined by X-ray crystallography. The Smad3-FoxH1 structure showed that FoxH1 bind to Smad3 using different mechanism from those of other Smad3 cofactors.

Report

(3 results)