Structural and functional analyses for the transcription factor complex in TGF-beta signalling.
| Project/Area Number |
15K14708
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Miyazono Kenichi 東京大学, 大学院農学生命科学研究科(農学部), 特任助教 (90554486)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | TGF-β / 転写因子 / X線結晶構造解析 / 構造解析 / 細胞分化 |
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| Outline of Final Research Achievements |
The transforming growth factor beta (TGF-beta) superfamily of cytokines regulates a number of biological processes such as cell self-renewal, differentiation, apoptosis and senescence. In this study, we focused on the transcription factor complex (Smad3-FoxH1) that is formed in cells by the stimulation of TGF-beta signal. The Smad3-FoxH1 complex regulates a number of gene expressions to regulate the differentiation of stem cells. Smad3 and FoxH1 from human were overexpressed in E. coli. The structure of Smad3-FoxH1 complex was determined by X-ray crystallography. The Smad3-FoxH1 structure showed that FoxH1 bind to Smad3 using different mechanism from those of other Smad3 cofactors.
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Report
(3 results)
Research Products
(3 results)
