Investigation and application of highly selective bromination reaction mechanism of biosynthetic enzyme of Tyrian purple, an ancient pigment
Project/Area Number |
15K14709
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
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Research Institution | Kanazawa University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
SAKURAI Takeshi 金沢大学, 物質化学系, 教授 (90116038)
HAYASHI Yoshihito 金沢大学, 物質化学系, 教授 (10231531)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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Keywords | bromoperoxidase / haloperoxidase / Tyrian purple / shellfish / ブロモペルオキシダーゼ / 臭素付加反応 / 貝紫色素 |
Outline of Final Research Achievements |
Tyrian purple having a molecular structure of 6,6'-dibromoindigo is a rare animal natural dye used since 1,600 BC. In this study, we tried to purify bromoperoxidase (BPO), which catalyzes the bromination reaction of purple pigment precursor from the hypobranchial gland of the shellfish, Rapana venosa. Since BPO exists only in trace amounts in shellfish, it could not be purified homogeneously, but revealed that a soluble activating protein is necessary for the activity of BPO present in the membrane fraction. BPO composed of two components is a novel enzyme, which has never been seen before.
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Report
(4 results)
Research Products
(4 results)
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[Journal Article] Heterologous Expression of Halomonas halodenitrificans Nitric Oxide Reductase and its N-Terminally Truncated NorC Subunit in Escherichia coli2017
Author(s)
Nobuhiko Sakurai, Kunishige Kataoka, Noriko Sugaya, Takaki Shimodaira, Mie Iwamoto, Munehiro Shoda, Hajime Horiuchi, Miyuki Kiyono, Yasuke Ohta, Banbang Triwiyono, Daisuke Seo, Takeshi Sakurai
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Journal Title
Journal of Inorganic Biochemistry
Volume: 169
Pages: 61-67
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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