Establishment of molecular basis on ligand recognition by the insect prothoracicotropic hormone receptor
| Project/Area Number |
15K14720
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Himeji Dokkyo University (2016)
The University of Tokyo (2015) |
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Principal Investigator |
SAITO Kazuki 姫路獨協大学, 薬学部, 教授 (10192585)
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| Co-Investigator(Renkei-kenkyūsha) |
KATAOKA Hiroshi 東京大学, 大学院新領域創成科学研究科, 教授 (60202008)
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| Research Collaborator |
KONOGAMI Tadafumi
YAMASHITA Yusuke
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 前胸腺刺激ホルモン（PTTH） / Torso / チロシンキナーゼ型レセプター / ジスルフィド架橋によるレセプター二量化 / カイコガ / 自己リン酸化 / 下流ERKリン酸化の促進 |
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| Outline of Final Research Achievements |
Insect prothoracicotropic hormone (PTTH) receptor, Torso, is a member of the receptor-tyrosine-kinase family. In this study, we detected ligand-independent dimerization of silkworm Torso, and found that the receptor molecules in the dimer were linked by intermolecular disulfide bridges in the transmembrane region. When all of the cysteines in the region were replaced with phenylalanines, non-covalent dimerization of the mutant was detected using a cross-linking reagent, both with and without ligand stimulation. This non-covalent dimerization caused apparent receptor autophosphorylation independently of the ligand stimulation, but did not promote the ERK phosphorylation in the downstream signaling pathway. The intermolecular disulfide bridges in the transmembrane region may play a key role in an activation process of the receptor, Torso.
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Report
(3 results)
Research Products
(15 results)
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[Presentation] 活性型ヨトウガリコンビナントPTTHの調製2017
Author(s)
山下雄佑, 此上祥史, 荻原麻理, 齋藤一樹, 片岡宏誌
Organizer
平成29年度蚕糸・昆虫機能利用学術講演会（日本蚕糸学会第87回大会）
Place of Presentation
農林水産技術会議事務局筑波産学連携支援センター（茨城県つくば市）
Year and Date
2017-03-22
Related Report
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