Chemical biology on cryptic genes activation
| Project/Area Number |
15K14721
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ケミカルバイオロジー / 天然物化学 / 複合培養 / 生物有機化学 / 生合成 / 抗真菌剤 / 抗生物質 / クリプティック遺伝子 |
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| Outline of Final Research Achievements |
During a high-throughput screening for identifying cell membrane modulators, eight novel 5-alkyl-1,2,3,4-tetrahydroquinolines (5aTHQs) bearing different side chains have been isolated from a combined-culture of Streptomyces sp. HEK616 and Tsukamurella pulmonis TP-B0596. The chemical structures including the absolute configuration were determined by spectroscopic analysis and total synthesis. Moreover, stable isotopic feeding experiments with sodium [1-13C]-acetate and [1,2-13C]-acetate in the combined-culture revealed that 5aTHQs is biosynthesized via a polyketide pathway. In addition, there novel streptoaminals were identified by an extensive mass spectrometry analysis against the combined-culture broth. These results suggest that the microbial combined-culture has a big potential for a range of chemical scaffolds.
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Report
(3 results)
Research Products
(19 results)
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[Presentation] 5-Alkyl-1,2,3,4-tetrahydroquinolines (5aTHQs), new membrane-interacting lipophilic metabolites, produced by combined-culture of Streptomyces nigrescens and Tsukamurella pulmonis2015
Author(s)
Sugiyama, R., Nishimura, S., Ozaki, T., Asamizu, S., Onaka, H., Kakeya, H.
Organizer
The International Chemical Congress of Pacific Basin Societies 2015 (PACIFICHEM 2015)
Place of Presentation
Hawaii, USA
Year and Date
2015-12-16
Related Report
Int'l Joint Research
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