| Project/Area Number |
15K14721
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ケミカルバイオロジー / 天然物化学 / 複合培養 / 生物有機化学 / 生合成 / 抗真菌剤 / 抗生物質 / クリプティック遺伝子 |
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| Outline of Final Research Achievements |
During a high-throughput screening for identifying cell membrane modulators, eight novel 5-alkyl-1,2,3,4-tetrahydroquinolines (5aTHQs) bearing different side chains have been isolated from a combined-culture of Streptomyces sp. HEK616 and Tsukamurella pulmonis TP-B0596. The chemical structures including the absolute configuration were determined by spectroscopic analysis and total synthesis. Moreover, stable isotopic feeding experiments with sodium [1-13C]-acetate and [1,2-13C]-acetate in the combined-culture revealed that 5aTHQs is biosynthesized via a polyketide pathway. In addition, there novel streptoaminals were identified by an extensive mass spectrometry analysis against the combined-culture broth. These results suggest that the microbial combined-culture has a big potential for a range of chemical scaffolds.
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