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Population genetic analysis for identifying hybrid origin of dwarf bamboo species using next-generation sequencing

Research Project

Project/Area Number 15K14745
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Forest science
Research InstitutionTohoku University

Principal Investigator

Suyama Yoshihisa  東北大学, 農学研究科, 准教授 (60282315)

Co-Investigator(Renkei-kenkyūsha) MAKITA AKIFUMI  秋田県立大学, 生物資源科学部, 教授 (60315596)
Research Collaborator YONEKURA KOJI  東北大学, 植物園, 助教
KANNO TAKAMASA  東北大学, 大学院農学研究科, 大学院生
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords雑種識別 / MIG-seq法 / 集団遺伝学 / NGS / ササ / ホンシャクナゲ / アズマザサ属
Outline of Final Research Achievements

Studies of the genetic origin of hybrids are required to clarify systematic relationships in many species groups. However, it is generally difficult to develop common DNA markers among different species for the genetic analysis. Here, we conducted a new approach, multiplexed ISSR genotyping by sequencing (MIG-seq) method, for identifying the parental species of two putative hybrid species (groups) of dwarf bamboo and Rhododendron. Our population genetic analysis strongly suggested hybrid origin of the species. This approach can be applied to investigating a wide range of hybrid species, and will be able to contribute to systematic studies of many species.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Inconsistency between morphological traits and ancestry of individuals in the hybrid zone between two Rhododendron japonoheptamerum varieties revealed by a genotyping-by-sequencing approach2017

    • Author(s)
      Ichiro Tamaki, Watanabe Yoichi, Yu Matsuki, Yoshihisa Suyama, Mizuo Mizuno
    • Journal Title

      Tree Genetics & Genomes

      Volume: 13 Issue: 1 Pages: 4-4

    • DOI

      10.1007/s11295-016-1084-x

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Improvement of MIG-seq: as an effective method for conservation genetics of Mangroves2016

    • Author(s)
      Yoshihisa Suyama, Yoshihiro Tsunamoto, Chika Mitsuyuki
    • Organizer
      The 3rd International Workshop for Conservation Genetics of Mangroves
    • Place of Presentation
      琉球大学熱帯生物学研究センター西表研究施設(沖縄県八重山郡竹富町)
    • Year and Date
      2016-10-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] MIG-seq: rapid SNP genotyping for conservation genetics using NGS2016

    • Author(s)
      Yoshihisa Suyama
    • Organizer
      The 7th EAFES International Congress
    • Place of Presentation
      Daegu(韓国)
    • Year and Date
      2016-04-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Population genetic analysis for identifying hybrid origin of a dwarf bamboo species in Sasaella2016

    • Author(s)
      Takamasa Kanno, Yoshihisa Suyama
    • Organizer
      The 13th International Symposium on Integrated Field Science: Conservation and Utilization of Biodiversity
    • Place of Presentation
      東北大学大学院農学研究科(宮城県仙台市)
    • Year and Date
      2016-03-09
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2018-03-22  

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