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Is the cyclicdiarylheptanoid signal compound for actinorhizal symbiosis

Research Project

Project/Area Number 15K14770
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Wood science
Research InstitutionShizuoka University

Principal Investigator

Kawai Shingo  静岡大学, 農学部, 教授 (70192549)

Co-Investigator(Kenkyū-buntansha) 米田 夕子  静岡大学, 農学部, 助教 (90638595)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsアクチノリザル共生 / 環状ジアリールヘプタノイド / 窒素固定 / ケミカルコミュニケーション / シグナル物質 / フランキア
Outline of Final Research Achievements

The Actinorhizal tree can preferentially acquire a nitrogen source by root nodule symbiosis. Therefore, identification of signal substances related to symbiosis with Alnus sieboldinana and Frankia and establishment of hydroponic cultivation method were carried out.
1. We investigated the influence of cyclic diarylheptanoids of Alnus sieboldiana on Frankia root nodule formation and clarified that these compounds markedly increase the number of nodules and are actinorhizal symbiotic signal substances. The compond was also confirmed by the LC-MS analysis in the roo extractst.
2. We have established a hydroponic cultivation that enables root growth during growth and observation of root nodule formation. However, compared to the soil cultivation, growth of plants and formation of nodules were not good. We concluded that the further examination of cultivation conditions is necessary.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 2015 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results) Remarks (1 results)

  • [Journal Article] Growth and nodulation in Alnus sieboldiana in response to Frankia inoculation and nitrogen treatments2016

    • Author(s)
      Takashi Yamanaka, Hiroaki Okabe, Shingo KawaiTakashi
    • Journal Title

      Tree

      Volume: 30 Issue: 2 Pages: 539-544

    • DOI

      10.1007/s00468-015-1246-8

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] オオバヤシャブシと放線菌フランキアの共生に関与する 抽出成分の検索(IV)2017

    • Author(s)
      櫻田明穂, 桑原遼太郎, 米田夕子, 河合真吾, 山中高史
    • Organizer
      第67回日本木材学会大会
    • Place of Presentation
      福岡県福岡市、九州大学及びアクロス福岡
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] オオバヤシャブシと放線菌フランキアの共生に関与する抽出成分の検索(III)2016

    • Author(s)
      櫻田明穂、米田夕子、山中高史、河合真吾
    • Organizer
      第66回日本木材学会大会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Cyclic diarylheptanoids act as signal compounds involved in symbiosis of nitrogen-fixing actinomycetes Frankia and Alnus sieboldiana2016

    • Author(s)
      Shingo Kawai, Akiho Sakurada, Takahiro Kaneko, Yuko Yoneda and Takashi Yamanaka
    • Organizer
      The 3rd International Workshop 2016 -Recent microbiological research for food, energy and health-
    • Place of Presentation
      タイ・バンコク・カセサート大学
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of Cyclic Diarylheptanoids about Alnus sieboldiana-Frankia Actinorhizal Symbiosis2015

    • Author(s)
      Shingo Kawai, Takahiro Kaneko, Yuko Yoneda, Tomoaki Nishida, Takashi Yamanaka
    • Organizer
      7th International Symposium on Indonesian Wood Research Society
    • Place of Presentation
      Grha Wiksa Praniti, Bandung, Indonesia
    • Year and Date
      2015-11-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Remarks] 静岡大学農学部森林生物化学研究室

    • URL

      http://www.agr.shizuoka.ac.jp/f/mokubake/index.html

    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2018-03-22  

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