Platform formation for pharmaceutical glycoprotein production system in wood rot fungus
| Project/Area Number |
15K14771
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Wood science
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| Research Institution | Kyoto University |
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Principal Investigator |
Honda Yoichi 京都大学, 農学研究科, 教授 (70252517)
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| Co-Investigator(Kenkyū-buntansha) |
中沢 威人 京都大学, 農学研究科, 助教 (80608141)
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| Research Collaborator |
NGYUEN DongXuan 京都大学, 大学院農学研究科, 院生
SAKAGUCHI Taku 京都大学, 大学院農学研究科, 院生
NISHISAKA Emi 京都大学, 大学院農学研究科, 院生
KODERA Rina 京都大学, 大学院農学研究科, 院生
SAKA Chinami 京都大学, 農学部森林科学科, 学生
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 白色腐朽菌 / 異種発現 / 糖タンパク質 / 遺伝子組換え / きのこ / 組換え / タンパク質 / 糖鎖 / 担子菌 / 木材腐朽菌 / タンパク質異種発現 / 組換え体 / タンパク質発現 |
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| Outline of Final Research Achievements |
With a transient gene expression system, that can evaluate gene expression level without nuisance of different gene dosage of integrated transgenes and their positional effect on the chromosome, it was demonstrated that a 14-bp stretch play a critical role in basic promoter function for beta-tubulin encoding gene in a white-rot fungus, Pleurotus ostreatus. The similar sequence was found among various basidiomycetes, suggesting that this stretch may serve as entry site for transcriptional factors as TATAA sequence does. A CT-rich sequence was also demonstrated to be important for the promoter function. Furthermore, a fusion gene encoding N-terminal of versatile peroxidase combined with luciferase was constructed and introduced in P. ostreatus. Activity of luciferase was detected in culture filtrate of this transformant, indicating that a reporter gene construct to investigate secretion system in wood-rot fungi was developed for the first time.
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Report
(4 results)
Research Products
(8 results)
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[Presentation] Yoichi Honda2017
Author(s)
Recent progress in molecular genetics of mushrooms
Organizer
9th Meeting of Asia for Mushroom Science
Related Report
Int'l Joint Research / Invited
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