| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The sterilization of artificial seeds of abalone is an ideal strategy to promote faster growth and to avoid sexual precocity. Recently, RNA interference (RNAi) is the popular method for the production of sterile animals in vertebrates and invertebrates, and identification of target gene for this technology is a crucial step to the production of sterilization. To evaluate effects of vasa gene knockdown, the dispersion and inhibitor effect of vasa dsRNA in ovary were examined. After 12 hours post-injection, dsRNA with vasa DIG-labelled delivered to full-grown oocytes of female. Expression levels of vasa in RNAi group using 3 different dose of dsRNA were significantly lower than control group at 7 days post-injection (dpi) and 14 dpi. Similar inhibitor effects were observed in unfertilized eggs and 8-cell stage of embryos. The results suggest that RNAi of vasa gene can be effectively utilized in oocytes and early embryos of pacific abalone to reduce amount of mRNA level.
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