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Development of maternal mRNA knock-down system using double-strand RNA in abalone embryos

Research Project

Project/Area Number 15K14802
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Aquatic life science
Research InstitutionKagoshima University (2016-2017)
Tokyo University of Marine Science and Technology (2015)

Principal Investigator

TAKEUCHI Yutaka  鹿児島大学, 農水産獣医学域水産学系, 助教 (70418680)

Research Collaborator YU Lingyun  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsアワビ / 胚発生 / vasa / RNA干渉 / qPCR / 卵巣 / 卵母細胞 / 生殖細胞 / dsRNA / RNA-seq / 産卵誘発 / 海産無脊椎動物 / アワビ類 / アワビ生殖腺 / トランスクリプト―ム解析 / dmc1 / 不妊化 / 不稔化
Outline of Final Research Achievements

The sterilization of artificial seeds of abalone is an ideal strategy to promote faster growth and to avoid sexual precocity. Recently, RNA interference (RNAi) is the popular method for the production of sterile animals in vertebrates and invertebrates, and identification of target gene for this technology is a crucial step to the production of sterilization. To evaluate effects of vasa gene knockdown, the dispersion and inhibitor effect of vasa dsRNA in ovary were examined. After 12 hours post-injection, dsRNA with vasa DIG-labelled delivered to full-grown oocytes of female. Expression levels of vasa in RNAi group using 3 different dose of dsRNA were significantly lower than control group at 7 days post-injection (dpi) and 14 dpi. Similar inhibitor effects were observed in unfertilized eggs and 8-cell stage of embryos. The results suggest that RNAi of vasa gene can be effectively utilized in oocytes and early embryos of pacific abalone to reduce amount of mRNA level.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Int'l Joint Research] Chinese Academy of Fishery Sciences/Marine Fishery Institute of Zhejiang(China)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Gonadal Transcriptome Analysis of Pacific Abalone Haliotis discus discus: Identification of Genes Involved in Germ Cell Development2018

    • Author(s)
      Lingyun Yu, Dongdong Xu, Huan Ye, Huamei Yue, Shioh Ooka, Hidehiro Kondo, Ryosuke Yazawa, Yutaka Takeuchi
    • Journal Title

      Marine Biotechnology

      Volume: 印刷中 Issue: 4 Pages: 467-480

    • DOI

      10.1007/s10126-018-9809-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Expression pattern of vasa gene in germ cells and vasa-dsRNA mediated RNA interference in oocytes and early embryos of pacific abalone (Haliotis discus discus)2017

    • Author(s)
      Lingyun Yu, Shioh Ooka, Huan Ye, Dongdong Xu, Hidehiro Kondo, Ryosuke Yazawa, Yutaka Takeuchi
    • Organizer
      International Symposium ”Fisheries Science for Future Generations"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gonadal transcriptome analysis of abalone (Haliotis discus discus): identification of genes involved in germ cell development2016

    • Author(s)
      Lingyun Yu・Huamei Yue・Shioh Ooka・Yasuko Ino・Ryosuke Yazawa・Yutaka Takeuchi
    • Organizer
      平成28年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Remarks] 鹿児島大学水産学部 水産資源科学分野(増養殖学コース)竹内裕研究室

    • URL

      https://www.facebook.com/Kagoshima.Univ.Takeuchi.Lab/

    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-05-15  

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