Isolation of of marine diatom-infecting virus promoters in the marine diatoms

• Project/Area Number: 15K14804
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Kochi University
• Principal Investigator: Adachi Masao 高知大学, 教育研究部自然科学系農学部門, 教授 (70274363)
• Co-Investigator(Renkei-kenkyūsha): TOMARU Yuji 水産研究・教育機構, 瀬戸内海区水産研究所, 主任研究員 (10416042)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 珪藻 / プロモーター / 形質転換 / ウイルス / 水圏バイオマス利用

Outline of Final Research Achievements

Transformation of diatom may enable us to solve the limitations in the mass production of beneficial chemicals for use in human activities. Promoter activity is important in genetic engineering. Thus, in this study, potential promoter regions were isolated from several marine diatom-infecting viruses (DIVs). DIV promoter-driving gene expression capacities in the Pennales diatom Phaeodactylum tricornutum were evaluated and compared to those of the endogenous promoter. We then examined the effects of the growth stage, the nutrient concentrations in media, and the photoperiods on viral promoter activity were investigated. Compared to diatom endogenous promoters, novel DIV promoter meditate a significantly higher degree of reporter transcription. Stable expression levels were observed in transformants grown under both light and dark conditions. This is the first report describing a promoter of DIVs that may be of use in basic and applied diatom research.

Research Products

• [Journal Article] Nuclear transformation of the diatom Phaeodactylum tricornutum using PCR-amplified DNA fragments by microparticle bombardment (2016)
  • Author(s): N. Kira, K. Ohnishi, A. Miyagawa-Yamaguchi, T. Kadono, and M. Adachi
  • Journal Title: Marine Genomics Volume: 25 Pages: 49-56
  • DOI: 10.1016/j.margen.2015.12.004
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 遺伝子改変海産珪藻を用いた有用物質生産に向けて (2016)
  • Author(s): 足立真佐雄・角野貴志
  • Journal Title: 化学工学 Volume: 80 Pages: 272-274
  • Peer Reviewed
• [Journal Article] Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum (2015)
  • Author(s): T. Kadono, A. Miyagawa-Yamaguchi, N. Kira, Y. Tomaru, T. Okami, T. Yoshimatsu, L. Hou, T. Ohama, K. Fukunaga, M. Okauchi, H. Yamaguchi, K. Ohnishi, A. Falciatore, and M. Adachi
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 18708-18708
  • DOI: 10.1038/srep18708
  • NAID: 120005983236
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Rhizosolenia属におけるメバロン酸経路関連遺伝子の発現解析 (2016)
  • Author(s): 角野貴志・大西裕美・福永一成・吉松孝倫・岡田茂・山口晴生・足立真佐雄
  • Organizer: 第３回分子珪藻研究会
  • Place of Presentation: ねぎや陵楓閣
  • Year and Date: 2016-12-18
• [Presentation] パーティクルガンを用いた珪藻の形質転換における直鎖状ベクターの有用性 (2016)
  • Author(s): 吉良望・大西浩平・山口（宮川）亜里沙・角野貴志・足立真佐雄
  • Organizer: 第３回分子珪藻研究会
  • Place of Presentation: ねぎや陵楓閣
  • Year and Date: 2016-12-18
• [Presentation] 海産珪藻Phaeodactylum tricornutumに由来する高発現型プロモーターの探索 (2016)
  • Author(s): 渡邉夢実・角野貴志・吉良望・鈴木健吾・岩田修・山口晴生・足立真佐
  • Organizer: 第３回分子珪藻研究会
  • Place of Presentation: ねぎや陵楓閣
  • Year and Date: 2016-12-18
• [Presentation] Effect of an introduced phytoene synthase gene expression on carotenoid biosynthesis in the marine diatom Phaeodactylum tricornutum (2015)
  • Author(s): T. Kadono, K. Suzuki, T. Ohama, S. Okada, M. Akakabe, M. Tsuda and M. Adachi
  • Organizer: Molecular Life of Diatoms 2015
  • Place of Presentation: University of Washington, Seatle, USA
  • Year and Date: 2015-07-07
  • Int'l Joint Research