| Project/Area Number |
15K14805
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic life science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KITANO Hajime 九州大学, 大学院農学研究院, 助教 (30635008)
MATSUYAMA Michiya 九州大学, 大学院農学研究院, 教授 (00183955)
NAGANO Naoki 九州大学, 大学院農学研究院, 准教授 (50437943)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | モデル生物 / カタクチイワシ / ゲノム編集 / 外来遺伝子発現 / ノックアウト / 遺伝子導入 / トランスジェニック魚 / バイオ医薬品 |
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| Outline of Final Research Achievements |
We focused on marine fish, the Japanese anchovy (Engraulis japonicas), as a candidate of a new model organism derived from teleosts. The breeding method of this species has been established. However, in order to utilize it as model organism, developing a system of genetic information and genetic manipulation is required. In this research, we established a microinjection system for the fertilized eggs and developed methods for high efficiency gene disruption via genome editing and foreign gene expression.
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