Development of high sencitive FISH for revealing functions of uncultured microorganisms
| Project/Area Number |
15K14807
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic life science
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| Research Institution | Anan National College of Technology |
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Principal Investigator |
Kawakami Shuji 阿南工業高等専門学校, 創造技術工学科, 講師 (00610461)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 環境微生物 / シングルセル解析 / FISH法 / aptamer / 微生物 / 排水処理 / アプタマー / 未培養微生物 |
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| Outline of Final Research Achievements |
In this study, we introduced an improved in situ DNA-HCR protocol (quickHCR-FISH) with higher sensitivity, and the amplification time was accelerated to only 15 min by using a dextran sulfate and blocking reagent containing amplification buffer, compared with 2 h in the original protocol. Finally, quickHCR-FISH was applied to marine bacteria in seawater and sediment samples for checking applicability of environmental microorganisms. The detection rates of marine bacteria after quickHCR-FISH were almost the same as after CARD-FISH. This newly developed protocol can be an attractive alternative to CARD-FISH for the detection of microorganisms in environmental context.
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Report
(3 results)
Research Products
(4 results)
