| Project/Area Number |
15K14837
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | インターフェロンτ / 子宮内膜細胞 / IFN tau / 構造ペプチド / 子宮上皮細胞 / interferon tau / ウシ / ウシ妊娠認識 |
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| Outline of Final Research Achievements |
The purpose of the present research is to search the activities of synthetic peptides constituting IFN-τ for a possible substitution for recombinant IFN-τ. Eleven peptides (long chain: 27-28 aa, short chain: 7-17 aa) have been chemically synthesized from amino acid sequence and structure of IFN-τ. Each or mixed peptides or recombinant IFN-τ were added to cultured bovine endometrium stromal cells to detect the expression of MX1, 2 and ISG15 that belong to interferon-stimulated genes (ISGs). Next, each or mixed peptides were added to the cells with recombinant IFN-τ and expression of ISGs was evaluated.IFN-τ significantly stimulated ISGs expression, whereas all single or mixed peptides did not. Addition of single or mixed peptides did not show the inhibitory effect of IFN-τ.These result suggest that synthetic peptides would not have agonist and antagonist ability or binding to IFN receptor by the possible structural change.
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