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Establishment of early pregnancy diagnosis using circulating exosomal microRNA in cows

Research Project

Project/Area Number 15K14838
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Animal production science
Research InstitutionIwate University

Principal Investigator

KIZAKI Keiichiro  岩手大学, 農学部, 教授 (40337994)

Co-Investigator(Renkei-kenkyūsha) TAKAHASHI Toru  岩手大学, 農学部, 教授 (20355738)
WATANABE Daisaku  北里大学, 獣医学部, 教授 (10406895)
IGA Kosuke  独立行政法人農業・食品産業技術総合研究機構, 東北農業研究センター, 主任研究員 (00343963)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsマイクロRNA / miRNA
Outline of Final Research Achievements

In the present study, we investigated the possibility of pregnancy diagnosis in cows using exosomal microRNA (miRNA) as a new biomarker. The present results demonstrated that some miRNAs were highly contained in pregnant plasma compared with non-pregnant. Furthermore, we identified also the reference miRNA for quantification of miRNA in plasma. These results indicate that measurement of selected miRNAs are reliable indicators of pregnancy in cows.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2017 2016

All Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] ウシ血中microRNAの発現プロファイルとリファレンス遺伝子の探索2017

    • Author(s)
      小野歌純,與座明祥,田路矩之,石黒(大沼)俊名,伊賀浩輔,木崎景一郎
    • Organizer
      第160回日本獣医学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Expression profile of microRNA in bovine endometrium at peri-implantation2017

    • Author(s)
      Yoza A, Toji N, Ishiguro-Oonuma T, Takahashi T, Hosoe M, Hashizume K, Kizaki K
    • Organizer
      The 4th World Congress of Reproductive Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ウシ栄養膜細胞系への効果的な遺伝子導入2016

    • Author(s)
      與座明祥,石黒(大沼)俊名,高橋透,橋爪一善,木崎景一郎
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Presentation] ウシ栄養膜に発現するmiRNAの探索2016

    • Author(s)
      與座明祥,石黒(大沼)俊名,高橋透,細江実佐,橋爪一善,木崎景一郎
    • Organizer
      第109回日本繁殖生物学会大会
    • Place of Presentation
      日本大学(神奈川県藤沢市)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
  • [Presentation] 血管新生制御因子バソヒビンのウシ子宮・胎盤における発現動態2016

    • Author(s)
      佐藤良平,石黒(大沼) 俊名,高橋透,橋爪一善,木崎 景一郎
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      麻布大学(神奈川県相模原市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Research-status Report
  • [Presentation] Effects of dietary forage and calf starter on ruminal pH and transcriptomic adaptation of rumen epithelium in Holstein calves during weaning transition2016

    • Author(s)
      Yohan Kim,渡部祐未,田路矩之,木崎景一郎,一條俊浩,佐藤 繁
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      日本大学(神奈川県藤沢市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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