Analysis of regulatory mechanism of ovulation in goats via establishment of conditional knockout animal model

• Project/Area Number: 15K14842
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Animal production science
• Research Institution: Nagoya University
• Principal Investigator: Ohkura Satoshi 名古屋大学, 生命農学研究科, 教授 (20263163)
• Co-Investigator(Renkei-kenkyūsha): MATSUDA Fuko 名古屋大学, 大学院生命農学研究科, 准教授 (10608855) ; HIRAO Yuji 農業・食品産業技術総合研究機構, 畜産草地研究所, 上席研究員 (10355349) ; AKAGI Satoshi 農業・食品産業技術総合研究機構, 畜産草地研究所, 主任研究員 (70414696)
• Research Collaborator: WATANABE Minami ; TATEBAYASHI Ryoki ; SUETOMI Yuta
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 応用動物 / 神経科学 / 獣医学 / 畜産学 / 性腺刺激ホルモン放出ホルモン / ヤギ / エストロジェン / 卵胞嚢腫 / GnRH

Outline of Final Research Achievements

In the present study, as the first step in establishing conditional esgrogen receptor (ER) gene (ESR1) knockout goats, we first revealed the base sequence of goat ESR1 mRNA and deduced amino acid sequence of goat ER. This information can be used for further determination of the goat ESR1 genomic structure and establishment of conditional knockout model. Second, we demonstrated that estrogen microimplants into the medial prepotic area (MPOA) induced surge-like luteinizing hormone (LH) release in gonadectomized male goat. These results suggest that the MPOA would be the brain mechanism controlling LH surge and plays a role in positive feedback actions on LH secretion in goats.

Research Products

• [Int'l Joint Research] フランス国立農業研究所(フランス)
• [Journal Article] The two populations of kisspeptin neurons are involved in the ram-induced LH pulsatile secretion and LH surge in anestrous ewes (2017)
  • Author(s): Fabre-Nys Claude、Cognie Juliette、Dufourny Laurence、Ghenim Meriem、Martinet Stephanie、Lasserre Olivier、Lomet Didier、Millar Robert P、Ohkura Satoshi、Suetomi Yuta
  • Journal Title: Endocrinology Volume: 158 Issue: 11 Pages: 3914-3928
  • DOI: 10.1210/en.2017-00429
  • Peer Reviewed
• [Journal Article] A neurokinin 3 receptor-selective agonist accelerates pulsatile luteinizing hormone secretion in lactating cattle† (2017)
  • Author(s): Nakamura Sho、Wakabayashi Yoshihiro、Yamamura Takashi、Ohkura Satoshi、Matsuyama Shuichi
  • Journal Title: Biology of Reproduction Volume: 97 Issue: 1 Pages: 81-90
  • DOI: 10.1093/biolre/iox068
  • Peer Reviewed
• [Journal Article] Immunohistochemical characterization of the arcuate kisspeptin/neurokinin B/dynorphin (KNDy) and preoptic kisspeptin neuronal populations in the hypothalamus during the estrous cycle in heifers. (2016)
  • Author(s): Hassaneen, A.S.A., Naniwa, Y., Suetomi, Y., Matsuyama, S., Kimura, K., Ieda, N., Inoue, N., Uenoyama, Y., Tsukamura, H., Maeda, K.-I., Matsuda, F. and Ohkura, S.
  • Journal Title: Journal of Reproduction and Development Volume: 62 Issue: 5 Pages: 471-477
  • DOI: 10.1262/jrd.2016-075
  • NAID: 130005431286
  • Peer Reviewed / Open Access
• [Journal Article] The Luteinising Hormone Surge-Generating System is Functional in Male Goats as in Females: Involvement of Kisspetin Neurons in the Medial Preoptic Area (2015)
  • Author(s): F. Matsuda, K. Nakatsukasa, Y. Suetomi, Y. Naniwa, D. Ito, N. Inoue, Y. Wakabayashi, H. Okamura, K.-I. Maeda, Y. Uenoyama, H. Tsukamura and S. Ohkura
  • Journal Title: Journal of Neuroendocrinology Volume: 27 Issue: 1 Pages: 57-65
  • DOI: 10.1111/jne.12235
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] κ-オピオイド受容体拮抗剤の末梢投与はシバヤギのパルス状LH分泌を亢進する. (2018)
  • Author(s): 佐々木拓弥・伊藤太祐・園田朋也・若林嘉浩・山村 崇・岡村裕昭・大石真也・藤井信孝・森田康広・上野山賀久・束村博子・前多敬一郎・松田二子・大蔵 聡
  • Organizer: 日本畜産学会第124回大会
• [Presentation] Microimplants of estradiol in the medial preoptic area induce a surge-like secretion of luteinizing hormone in male Shiba goats. (2017)
  • Author(s): Watanabe, M., Sasaki, T., Tatebayashi, R., Suetomi, Y. and Ohkura, S.
  • Organizer: The 4th World Congress on Reproductive Biology
  • Int'l Joint Research
• [Presentation] Peripheral administration of κ-opioid receptor antagonist stimulates pulsatile LH secretion by acting on the GnRH pulse generator activity in goats. (2017)
  • Author(s): Sasaki, T., Ito, D., Sonoda, T., Wakabayashi, Y., Yamamura, T., Okamura, H., Oishi, S., Noguchi, T., Fujii, N., Uenoyama, Y. Tsukamura, H., Maeda, K.-I., Matsuda, F. and Ohkura, S.
  • Organizer: The 4th World Congress on Reproductive Biology
  • Int'l Joint Research
• [Presentation] A neurokinin 3 receptor-selective agonist, senktide, stimulates pulsatile luteinizing hormone secretion in lactating cattle. (2017)
  • Author(s): Nakamura, S., Wakabayashi, Y., Yamamura, T., Ohkura, S. and Matsuyama, S.
  • Organizer: The 4th World Congress on Reproductive Biology
  • Int'l Joint Research
• [Presentation] Stimulation of the GnRH pulse generator activity by peripheral administration of a κ-opioid receptor antagonist in goats. (2017)
  • Author(s): Sasaki, T., Ito, D., Sonoda, T., Wakabayashi, Y., Yamamura, T., Okamura, H., Oishi, S., Noguchi, T., Fujii, N., Uenoyama, Y. Tsukamura, H., Maeda, K.-I., Matsuda, F. and Ohkura, S.
  • Organizer: Society for Neuroscience 47th Annual Meeting
  • Int'l Joint Research
• [Presentation] 泌乳期ウシにおけるニューロキニンB受容体作動薬が黄体形成ホルモンのパルス状分泌に及ぼす影響. (2017)
  • Author(s): 中村 翔・若林嘉浩・山村 崇・大蔵 聡・松山秀一
  • Organizer: 日本下垂体研究会第32回学術集会
• [Presentation] Modification of KISS1 gene in goat embryonic fibroblasts using TALEN (2016)
  • Author(s): Tatebayashi, R., Sakuma, T., Yamamoto, T., Ohkura, S. and Matsuda, F.
  • Organizer: International Symposium on Pituitary Gland and Its Related Systems
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] 排卵中枢キスペプチンニューロンの性分化には種差がある (2016)
  • Author(s): 松田二子・渡辺雄貴・大蔵 聡・井上直子・上野山賀久・前多敬一郎・束村博子
  • Organizer: 第13回GPCR研究会
  • Place of Presentation: 東京都江東区
  • Year and Date: 2016-05-13
• [Presentation] アデノ随伴ウイルス（AAV）ベクターを用いたヤギ視床下部神経細胞への遺伝子導入法の検討 (2015)
  • Author(s): 末富祐太・奥田雄大・小林憲太・大蔵 聡・松田二子
  • Organizer: 第108回日本繁殖生物学会大会
  • Place of Presentation: 宮崎県宮崎市
  • Year and Date: 2015-09-17
• [Presentation] TALENを用いたシバヤギ体細胞のKISS1遺伝子改変 (2015)
  • Author(s): 舘林亮輝・佐久間哲史・山本 卓・大蔵 聡・松田二子
  • Organizer: 第108回日本繁殖生物学会大会
  • Place of Presentation: 宮崎県宮崎市
  • Year and Date: 2015-09-17
• [Book] Masterclass in Neuroendocrinology (2017)
  • Author(s): Goodman, R.L., Okhura, S., Okamura, H., Coolen, L.M. and Lehman, M.N.
  • Publisher: John Wiley & Sons, Inc.
• [Remarks] 名古屋大学大学院生命農学研究科 動物生産科学研究室
  • URL: http://www.agr.nagoya-u.ac.jp/~laps/index-j.html
• [Remarks] 名古屋大学大学院生命農学研究科 動物生産科学第1研究分野
  • URL: http://www.agr.nagoya-u.ac.jp/~laps/index-j.html